RRC ID 30041
著者 Hill CW, Harnish BW.
タイトル Inversions between ribosomal RNA genes of Escherichia coli.
ジャーナル Proc Natl Acad Sci U S A
Abstract It might be anticipated that the presence of redundant but oppositely oriented sequences in a chromosome could allow inversion of the intervening material through homologous recombination. For example, the ribosomal RNA gene rrnD of Escherichia coli has the opposite orientation fro rrnB and rrnE and is separated from these genes by roughly 20% of the chromosome. Starting with a derivative of Cavalli Hfr, we have constructed mutants that have an inversion of the segment between rrnD and either rrnB or rrnE. These mutants are generally quite viable but do exhibit a slight reduction in growth rate relative to the parental strain. A major line of laboratory E. coli, W3110 and its derivatives, also has an inversion between rrnD and rrnE, probably created directly by a recombinational event between these highly homologous genes.
巻・号 78(11)
ページ 7069-72
公開日 1981-11-1
DOI 10.1073/pnas.78.11.7069
PMID 6273909
PMC PMC349196
MeSH Crosses, Genetic DNA Restriction Enzymes Escherichia coli / genetics* Mutation Nucleic Acid Hybridization RNA, Ribosomal / genetics* Recombination, Genetic Suppression, Genetic*
IF 9.412
引用数 230
WOS 分野 BIOLOGY
リソース情報
原核生物(大腸菌) ME6253