論文 - 詳細
RRC ID | 30053 |
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著者 | Brooks JE, Blumenthal RM, Gingeras TR. |
タイトル | The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene. |
ジャーナル | Nucleic Acids Res |
Abstract |
The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning experiments established a set of approximate boundaries of the gene. The nucleotide sequence of the dam gene was determined, and analysis of that sequence revealed a unique open reading frame which corresponded in length to that necessary to code for a protein the size of dam. Amino acid composition derived from this sequence corresponds closely to the amino acid composition of the purified dam protein. Enzymatic and DNA:DNA hybridization methods were used to investigate the possible presence of dam genes in a variety of prokaryotic organisms. |
巻・号 | 11(3) |
ページ | 837-51 |
公開日 | 1983-2-11 |
DOI | 10.1093/nar/11.3.837 |
PMID | 6300769 |
PMC | PMC325756 |
MeSH | Amino Acid Sequence Base Sequence Cloning, Molecular* DNA (Cytosine-5-)-Methyltransferases / genetics* DNA Restriction Enzymes DNA, Bacterial / genetics DNA, Bacterial / isolation & purification* Escherichia coli / enzymology* Genes* Genes, Bacterial* Methyltransferases / genetics* Nucleic Acid Hybridization Plasmids |
IF | 11.502 |
引用数 | 152 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
原核生物(大腸菌) | ME9000 |