論文 - 詳細
| RRC ID | 30072 |
|---|---|
| 著者 | Brass JM, Manson MD, Larson TJ. |
| タイトル | Transposon Tn10-dependent expression of the lamB gene in Escherichia coli. |
| ジャーナル | J Bacteriol |
| Abstract |
Among Tn10 insertions isolated in or near the malB region of Escherichia coli, one (zjb-729::Tn10) mapped between malK and lamB or late in malK and allowed MalT-independent expression of lamB. Tn10-dependent expression of a lamB-lacZ protein fusion was 25% of the expression of the fusion from the malK-lamB operon promoter in malTc constitutive strains. The maltoporin content of a strain carrying this Tn10 was about 20% that of a malTc malB+ strain. Transport of maltose at concentrations of below 10(-6) M was reduced about threefold. When maltoporin was present at about 50% of the level of malTc malB+ strains, maltose transport was largely restored. We conclude that maltoporin is not rate limiting for maltose transport in wild-type cells but becomes rate limiting when the ratio of maltoporin to other maltose transport components is reduced more than twofold. |
| 巻・号 | 159(1) |
| ページ | 93-9 |
| 公開日 | 1984-7-1 |
| DOI | 10.1128/jb.159.1.93-99.1984 |
| PMID | 6330053 |
| PMC | PMC215597 |
| MeSH | Bacterial Outer Membrane Proteins Biological Transport Chromosomes, Bacterial / physiology Coliphages / genetics DNA Restriction Enzymes DNA Transposable Elements* Escherichia coli / genetics* Escherichia coli / growth & development Genes* Genes, Bacterial* Genotype Maltose / metabolism Porins Receptors, Virus / genetics* Species Specificity beta-Galactosidase / genetics |
| IF | 3.006 |
| 引用数 | 26 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | ME8464 |