RRC ID 30150
著者 Yasuda T, Morimatsu K, Horii T, Nagata T, Ohmori H.
タイトル Inhibition of Escherichia coli RecA coprotease activities by DinI.
ジャーナル EMBO J
Abstract In Escherichia coli, the SOS response is induced upon DNA damage and results in the enhanced expression of a set of genes involved in DNA repair and other functions. The initial step, self-cleavage of the LexA repressor, is promoted by the RecA protein which is activated upon binding to single-stranded DNA. In this work, induction of the SOS response by the addition of mitomycin C was found to be prevented by overexpression of the dinI gene. dinI is an SOS gene which maps at 24.6 min of the E.coli chromosome and encodes a small protein of 81 amino acids. Immunoblotting analysis with anti-LexA antibodies revealed that LexA did not undergo cleavage in dinI-overexpressed cells after UV irradiation. In addition, the RecA-dependent conversion of UmuD to UmuD' (the active form for mutagenesis) was also inhibited in dinI-overexpressed cells. Conversely, a dinI-deficient mutant showed a slightly faster and more extensive processing of UmuD and hence higher mutability than the wild-type. Finally, we demonstrated, by using an in vitro reaction with purified proteins, that DinI directly inhibits the ability of RecA to mediate self-cleavage of UmuD.
巻・号 17(11)
ページ 3207-16
公開日 1998-6-1
DOI 10.1093/emboj/17.11.3207
PMID 9606202
PMC PMC1170659
MeSH Bacterial Proteins / biosynthesis Bacterial Proteins / genetics* Bacterial Proteins / metabolism DNA-Directed DNA Polymerase Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial* Mutagenesis Rec A Recombinases / antagonists & inhibitors* Rec A Recombinases / genetics Rec A Recombinases / metabolism Recombination, Genetic SOS Response, Genetics / genetics*
IF 9.889
引用数 61
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
原核生物(大腸菌) ME9625 ME9626 ME9627