RRC ID 30725
著者 Matsumoto S, Fujii S, Sato A, Ibuka S, Kagawa Y, Ishii M, Kikuchi A.
タイトル A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures.
ジャーナル EMBO J
Abstract Growth factor-dependent epithelial morphological changes and proliferation are essential for the formation of tubular structures, but the underlying molecular mechanisms are poorly understood. Co-stimulation with Wnt3a and epidermal growth factor (Wnt3a/EGF) induced development of tubes consisting of intestinal epithelial cells by inducing expression of Arl4c, an Arf-like small GTP-binding protein, in three-dimensional culture, while stimulation with Wnt3a or EGF alone did not. Arl4c expression resulted in rearrangement of the cytoskeleton through activation of Rac and inactivation of Rho properly, which promoted cell growth by inducing nuclear translocation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif (YAP/TAZ) in leading cells. Arl4c was expressed in ureteric bud tips and pretubular structures in the embryonic kidney. In an organoid culture assay, Wnt and fibroblast growth factor signaling simultaneously induced elongation and budding of kidney ureteric buds through Arl4c expression. YAP/TAZ was observed in the nucleus of extending ureteric bud tips. Thus, Arl4c expression induced by a combination of growth factor signaling mechanisms is involved in tube formation.
巻・号 33(7)
ページ 702-18
公開日 2014-4-1
DOI 10.1002/embj.201386942
PII embj.201386942
PMID 24562386
PMC PMC4000088
MeSH ADP-Ribosylation Factors / metabolism* Acyltransferases Animals Cell Adhesion Cell Differentiation Cells, Cultured Cytoskeleton / metabolism Dogs Epidermal Growth Factor / metabolism* Epithelial Cells / cytology Epithelial Cells / physiology Female Fetus Fibroblast Growth Factors / metabolism HeLa Cells Humans Intestinal Mucosa / metabolism Intestines / cytology Kidney / embryology Madin Darby Canine Kidney Cells Mice Mice, Inbred C57BL Rats Signal Transduction* Transcription Factors / metabolism* Wnt3 Protein / metabolism*
IF 9.889
引用数 41
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
遺伝子材料 B6N BAC Mouse (RDB07573) B6Ng01-089N24 CSII-EF-MCS (RDB04378) CSII-CMV-MCS-IRES2-Bsd (RDB04385) CS-RfA-EVBsd (RDB06090) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394).