RRC ID |
30905
|
著者 |
Hashimoto M, Aruga J, Hosoya Y, Kanegae Y, Saito I, Mikoshiba K.
|
タイトル |
A neural cell-type-specific expression system using recombinant adenovirus vectors.
|
ジャーナル |
Hum Gene Ther
|
Abstract |
We demonstrated neural cell-type-specific gene expression using an adenovirus vector, which is useful for delivering foreign genes into quiescent neural cells. We produced eight recombinant replication-defective adenoviruses carrying the lacZ reporter gene driven by various promoters, including those of the L7/PCP2 gene (highly restricted expression in cerebellar Purkinje cells), and the myelin basic protein (in oligodendrocytes) gene. We demonstrated in vitro and in vivo promoter-driven, neural cell-type-specific gene expression by recombinant adenoviruses. The genes were transferred into these recombinant adenoviruses and expressed with high levels of efficiency in vitro and in vivo. In primary culture, the recombinant adenoviruses AdexL7-NL-LacZ and AdexMBP-NL-LacZ appeared to be expressed in a Purkinje cell and oligodendrocyte-specific manner. Introduction of 10(8) pfu of these viruses into the adult rat cerebellum by stereotactic injection yielded neural cell-type-specific expression without apparent toxicity to the animals. Thus, adenovirus vectors are useful for cell-type-specific therapeutic uses and in studies requiring neural cell-type-specific gene expression.
|
巻・号 |
7(2)
|
ページ |
149-58
|
公開日 |
1996-1-20
|
DOI |
10.1089/hum.1996.7.2-149
|
PMID |
8788166
|
MeSH |
Adenoviruses, Human / genetics*
Animals
Cells, Cultured
Cerebellum / cytology
Female
Gene Expression*
Gene Transfer Techniques*
Genes, Reporter / genetics
Genetic Vectors*
Humans
Lac Operon / genetics
Mice
Mice, Inbred ICR
Myelin Basic Protein / biosynthesis
Myelin Basic Protein / genetics
Nerve Tissue Proteins / genetics
Oligodendroglia / physiology
Purkinje Cells / physiology*
Rats
Rats, Sprague-Dawley
Recombinant Fusion Proteins / biosynthesis
|
IF |
4.51
|
引用数 |
72
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
MEDICINE, RESEARCH & EXPERIMENTAL
|
リソース情報 |
遺伝子材料 |
pAdex L7 (RDB06009)
pAdex L7-NL-Cre (RDB06010)
Adex L7-NL-Cre (RDB06011)
pAdex MBP-SwaI-SV40pA (RDB06014) |