論文 - 詳細
| RRC ID | 31989 |
|---|---|
| 著者 | Aomatsu E, Takahashi N, Sawada S, Okubo N, Hasegawa T, Taira M, Miura H, Ishisaki A, Chosa N. |
| タイトル | Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells. |
| ジャーナル | Sci Rep |
| Abstract |
Human mesenchymal stem cells (hMSCs) remodel or regenerate various tissues through several mechanisms. Here, we identified the hMSC-secreted protein SCRG1 and its receptor BST1 as a positive regulator of self-renewal, migration, and osteogenic differentiation. SCRG1 and BST1 gene expression decreased during osteogenic differentiation of hMSCs. Intriguingly, SCRG1 maintained stem cell marker expression (Oct-4 and CD271/LNGFR) and the potentials of self-renewal, migration, and osteogenic differentiation, even at high passage numbers. Thus, the novel SCRG1/BST1 axis determines the fate of hMSCs by regulating their kinetic and differentiation potentials. Our findings provide a new perspective on methods for ex vivo expansion of hMSCs that maintain native stem cell potentials for bone-forming cell therapy. |
| 巻・号 | 4 |
| ページ | 3652 |
| 公開日 | 2014-1-13 |
| DOI | 10.1038/srep03652 |
| PII | srep03652 |
| PMID | 24413464 |
| PMC | PMC3888969 |
| MeSH | ADP-ribosyl Cyclase / genetics* ADP-ribosyl Cyclase / metabolism Antigens, CD / genetics* Antigens, CD / metabolism Cell Differentiation / genetics* Cell Movement / genetics Down-Regulation Extracellular Signal-Regulated MAP Kinases / metabolism Focal Adhesion Protein-Tyrosine Kinases / metabolism GPI-Linked Proteins / genetics GPI-Linked Proteins / metabolism Gene Expression Regulation, Developmental Humans JNK Mitogen-Activated Protein Kinases / metabolism Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / metabolism* Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Osteogenesis / genetics* Phosphatidylinositol 3-Kinases / metabolism Protein Binding Protein Biosynthesis Signal Transduction |
| IF | 3.998 |
| 引用数 | 28 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 遺伝子材料 | pSCRG1-FLAG (RDB12800) |