RRC ID 3251
著者 Sato Y, Kumazawa N, Yoshikawa K, Kurusu Y.
タイトル Transformation of Escherichia coli mediated by natural phospholipids.
ジャーナル Biosci Biotechnol Biochem
Abstract Transformation system for Escherichia coli based upon introduction of plasmid DNA by natural phospholipids has been developed. Transformants are easily obtained by treatment with natural phospholipids such as phosphatidylethanolamine, phosphatidylcoline, and phosphatidylserine, where the presence of MgCl2 or CaCl2 is essential. This method of transformation is applicable not only for small plasmid pHSG399 (2.3 Kb) but also for giant plasmid R6K (100 Kb).
巻・号 69(1)
ページ 235-7
公開日 2005-1-1
DOI 10.1271/bbb.69.235
PII JST.JSTAGE/bbb/69.235
PMID 15665495
MeSH DNA, Bacterial / genetics Escherichia coli / drug effects Escherichia coli / genetics* Phosphatidylethanolamines / pharmacology* Plasmids* Transformation, Bacterial*
IF 1.516
引用数 9
WOS 分野 CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME8568(HB101)