論文 - 詳細
RRC ID | 3251 |
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著者 | Sato Y, Kumazawa N, Yoshikawa K, Kurusu Y. |
タイトル | Transformation of Escherichia coli mediated by natural phospholipids. |
ジャーナル | Biosci Biotechnol Biochem |
Abstract |
Transformation system for Escherichia coli based upon introduction of plasmid DNA by natural phospholipids has been developed. Transformants are easily obtained by treatment with natural phospholipids such as phosphatidylethanolamine, phosphatidylcoline, and phosphatidylserine, where the presence of MgCl2 or CaCl2 is essential. This method of transformation is applicable not only for small plasmid pHSG399 (2.3 Kb) but also for giant plasmid R6K (100 Kb). |
巻・号 | 69(1) |
ページ | 235-7 |
公開日 | 2005-1-1 |
DOI | 10.1271/bbb.69.235 |
PII | JST.JSTAGE/bbb/69.235 |
PMID | 15665495 |
MeSH | DNA, Bacterial / genetics Escherichia coli / drug effects Escherichia coli / genetics* Phosphatidylethanolamines / pharmacology* Plasmids* Transformation, Bacterial* |
IF | 1.516 |
引用数 | 9 |
WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
原核生物(大腸菌) | ME8568(HB101) |