論文 - 詳細
| RRC ID | 32576 |
|---|---|
| 著者 | Yamada H, Kikuchi S, Inui T, Takahashi H, Kimura K. |
| タイトル | Gentiolactone, a secoiridoid dilactone from Gentiana triflora, inhibits TNF-α, iNOS and Cox-2 mRNA expression and blocks NF-κB promoter activity in murine macrophages. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Gentian roots have been used as a herbal medicine because of their anti-inflammatory activities. However, the molecular mechanisms of these anti-inflammatory effects remain to be completely explained. METHODS AND FINDINGS:Here, we investigated anti-inflammatory effects of gentian roots and showed that root extracts from Gentiana triflora inhibited lipopolysaccharide (LPS)-induced expression of TNF-α in RAW264.7 cells. The extracts also contained swertiamarin and gentiopicroside, which are the major active compounds of gentian roots; however, neither compound had any effect on LPS-induced TNF-α production in our test system. We isolated gentiolactone as an inhibitor of TNF-α production from the extracts. Gentiolactone also inhibited LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) expression at the mRNA level. Moreover, gentiolactone suppressed NF-κB transcriptional activity without inhibition of IκB degradation or NF-κB nuclear transport. CONCLUSIONS:Our results indicate that inhibition of TNF-α, iNOS and Cox-2 expression by gentiolactone is one of the mechanisms of the anti-inflammatory properties of gentian roots. |
| 巻・号 | 9(11) |
| ページ | e113834 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0113834 |
| PII | PONE-D-14-21637 |
| PMID | 25423092 |
| PMC | PMC4244148 |
| MeSH | Animals Cyclooxygenase 2 / genetics* Gentiana / chemistry* Iridoids / pharmacology* Macrophages / drug effects* Macrophages / metabolism Mice NF-kappa B / genetics* Nitric Oxide Synthase Type II / genetics* Promoter Regions, Genetic* RNA, Messenger / genetics* Tumor Necrosis Factor-alpha / genetics* |
| IF | 2.74 |
| 引用数 | 11 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| 遺伝子材料 | pNF-kappaB RE-TK hRluc(F) (RDB05576) |