| RRC ID |
32711
|
| 著者 |
Garciandia A, Suarez T.
|
| タイトル |
The NMRA/NMRAL1 homologue PadA modulates the expression of extracellular cAMP relay genes during aggregation in Dictyostelium discoideum.
|
| ジャーナル |
Dev Biol
|
| Abstract |
NMRA-like proteins belong to a class of conserved transcriptional regulators that function as direct sensors of the metabolic state of the cell and link basic metabolism to changes in gene expression. PadA was the first NMRA-like protein described in Dictyostelium discoideum and was shown to be necessary for prestalk cell differentiation and correct development. We describe and characterize padA(-) mutant phenotype during the onset of development, which results in the formation of abnormally small territories and impairment of cAMP responses. Transcriptional analysis shows that cAMP-induced gene expression is downregulated in padA(-), particularly the genes that establish the extracellular cAMP relay. The mutant phenotype can be rescued with the constitutive expression of one of these genes, carA, encoding the cAMP receptor. Transcriptional analysis of padA(-)/A15::carA showed that carA maximum mRNA levels were not reached during aggregation. Our data support a regulatory role for PadA on the regulation of extracellular cAMP relay genes during aggregation and suggest that PadA is required to achieve carA full induction.
|
| 巻・号 |
381(2)
|
| ページ |
411-22
|
| 公開日 |
2013-9-15
|
| DOI |
10.1016/j.ydbio.2013.06.013
|
| PII |
S0012-1606(13)00330-8
|
| PMID |
23773804
|
| MeSH |
Cell Adhesion
Chemotaxis
Cyclic AMP / genetics
Cyclic AMP / metabolism*
Dictyostelium / genetics
Dictyostelium / growth & development
Dictyostelium / metabolism*
Gene Expression Regulation, Developmental
Genes, Protozoan*
Phenotype
Protein Interaction Mapping
Protozoan Proteins / genetics
Protozoan Proteins / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Receptors, Cyclic AMP / genetics
Receptors, Cyclic AMP / metabolism
Regulatory Elements, Transcriptional
Transcription, Genetic
|
| IF |
2.896
|
| 引用数 |
6
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
G01319 |
