RRC ID |
33187
|
著者 |
Fujii T, Abe H, Kawamoto M, Banno Y, Shimada T.
|
タイトル |
Positional cloning of the sex-linked giant egg (Ge) locus in the silkworm, Bombyx mori.
|
ジャーナル |
Insect Mol Biol
|
Abstract |
The giant egg (Ge) locus is a Z-linked mutation that leads to the production of large eggs. Cytological observations suggest that an unusual translocation of a large fragment of the W chromosome bearing a putative egg size-determining gene, Esd, gave rise to giant egg mutants. However, there is currently no molecular evidence confirming either a W-Z translocation or the presence of Esd on the W chromosome. To elucidate the origin of giant egg mutants, we performed positional cloning. We observed that the Bombyx mori. orthologue of the human Phytanoyl-CoA dioxygenase domain containing 1 gene (PHYHD1) is disrupted in giant egg mutants. PHYHD1 is highly conserved in eukaryotes and is predicted to be a Fe(II) and 2-oxoglutarate-dependent oxygenase. Exon skipping in one of the two available Ge mutants is probably caused by the insertion of a non-long terminal repeat transposon into intron 4 in the vicinity of the 5' splice site. Segmental duplication in Ge(2) , an independent allele, was caused by unequal recombination between short interspersed elements inserted into introns 3 and 5. Our results indicate that (1) Bombyx PHYHD1 is responsible for the Ge mutants and that (2) the Ge locus is unrelated to the W-linked putative Esd. To our knowledge, this is the first report describing the phenotypic defects caused by mutations in PHYHD1 orthologues.
|
巻・号 |
24(2)
|
ページ |
213-21
|
公開日 |
2015-4-1
|
DOI |
10.1111/imb.12150
|
PMID |
25469867
|
MeSH |
Animals
Base Sequence
Bombyx / genetics*
Chromosome Mapping
Cloning, Molecular
Female
Genetic Loci*
Genetic Techniques
Molecular Sequence Data
Mutation
Oogenesis / genetics
Ovum / cytology
Sex Chromosomes / genetics*
|
IF |
2.533
|
引用数 |
3
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
ENTOMOLOGY
|
リソース情報 |
カイコ |
d20
d21
l83
k151
n70 |