RRC ID 3327
著者 Miyakawa T, Sawano Y, Miyazono K, Hatano K, Tanokura M.
タイトル Crystallization and preliminary X-ray analysis of ginkbilobin-2 from Ginkgo biloba seeds: a novel antifungal protein with homology to the extracellular domain of plant cysteine-rich receptor-like kinases.
ジャーナル Acta Crystallogr Sect F Struct Biol Cryst Commun
Abstract The antifungal protein ginkbilobin-2 (Gnk2) from Ginkgo biloba seeds does not show homology to other pathogenesis-related proteins, but does show homology to the extracellular domain of plant cysteine-rich receptor-like kinases. Native Gnk2 purified from ginkgo nuts and the selenomethionine derivative of recombinant Gnk2 (SeMet-rGnk2) were crystallized by the sitting-drop vapour-diffusion method using different precipitants. X-ray diffraction data were collected from Gnk2 at 2.38 A resolution and from SeMet-rGnk2 at 2.79 A resolution using a synchrotron-radiation source. The crystals of both proteins belonged to the primitive cubic space group P2(1)3, with unit-cell parameters a = b = c = 143.2 A.
巻・号 63(Pt 9)
ページ 737-9
公開日 2007-9-1
DOI 10.1107/S1744309107034793
PII S1744309107034793
PMID 17768341
PMC PMC2376327
MeSH Antimicrobial Cationic Peptides / chemistry* Antimicrobial Cationic Peptides / isolation & purification Crystallization Cysteine Ginkgo biloba Plant Proteins / chemistry Plant Proteins / isolation & purification Plants / enzymology Protein Kinases / chemistry Protein Kinases / isolation & purification Seeds / chemistry* X-Ray Diffraction
IF 0.968
引用数 9
WOS 分野 CRYSTALLOGRAPHY BIOPHYSICS BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME7982 (JM109 pDsbCD1)