論文 - 詳細
RRC ID | 3336 |
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著者 | Smith LK, Gomez MJ, Shatalin KY, Lee H, Neyfakh AA. |
タイトル | Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes. |
ジャーナル | Genome Biol |
Abstract |
We have developed a new microarray-based genetic technique, named MGK (Monitoring of Gene Knockouts), for genome-wide identification of conditionally essential genes. MGK identified bacterial genes that are critical for fitness in the absence of aromatic amino acids, and was further applied to identify genes whose inactivation causes bacterial cell death upon exposure to the bacteriostatic antibiotic chloramphenicol. Our findings suggest that MGK can serve as a robust tool in functional genomics studies. |
巻・号 | 8(5) |
ページ | R87 |
公開日 | 2007-1-1 |
DOI | 10.1186/gb-2007-8-5-r87 |
PII | gb-2007-8-5-r87 |
PMID | 17519022 |
PMC | PMC1929150 |
MeSH | Anti-Bacterial Agents / pharmacology Bacteria / drug effects Bacteria / genetics Bacteria / growth & development* Chloramphenicol / pharmacology Gene Deletion Gene Silencing* Genes, Bacterial / physiology* Genome, Bacterial Genomics / methods |
IF | 10.806 |
引用数 | 7 |
WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
リソース情報 | |
原核生物(大腸菌) | ME9062(BW25113) |