RRC ID 33535
著者 Yoshida KI, Fujita Y, Ehrlich SD.
タイトル An operon for a putative ATP-binding cassette transport system involved in acetoin utilization of Bacillus subtilis.
ジャーナル J Bacteriol
Abstract The ytrABCDEF operon of Bacillus subtilis was deduced to encode a putative ATP-binding cassette (ABC) transport system. YtrB and YtrE could be the ABC subunits, and YtrC and YtrD are highly hydrophobic and could form a channel through the cell membrane, while YtrF could be a periplasmic lipoprotein for substrate binding. Expression of the operon was examined in cells grown in a minimal medium. The results indicate that the expression was induced only early in the stationary phase. The six ytr genes form a single operon, transcribed from a putative sigma(A)-dependent promoter present upstream of ytrA. YtrA, which possesses a helix-turn-helix motif of the GntR family, acts probably as a repressor and regulates its own transcription. Inactivation of the operon led to a decrease in maximum cell yield and less-efficient sporulation, suggesting its involvement in the growth in stationary phase and sporulation. It is known that B. subtilis produces acetoin as an external carbon storage compound and then reuses it later during stationary phase and sporulation. When either the entire ytr operon or its last gene, ytrF, was inactivated, the production of acetoin was not affected, but the reuse of acetoin became less efficient. We suggest that the Ytr transport system plays a role in acetoin utilization during stationary phase and sporulation.
巻・号 182(19)
ページ 5454-61
公開日 2000-10-1
DOI 10.1128/JB.182.19.5454-5461.2000
PMID 10986249
PMC PMC110989
MeSH ATP-Binding Cassette Transporters / genetics* Acetoin / metabolism* Adenosine Triphosphate / metabolism* Bacillus subtilis / genetics* Bacillus subtilis / growth & development Bacillus subtilis / metabolism Bacterial Proteins / genetics* Base Sequence Biological Transport Gene Expression Genes, Bacterial* Molecular Sequence Data Operon* Repressor Proteins / genetics
IF 3.006
引用数 37
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(枯草菌) MBS212