論文 - 詳細
| RRC ID | 33578 |
|---|---|
| 著者 | Fujita Y, Fujita T. |
| タイトル | Identification and nucleotide sequence of the promoter region of the Bacillus subtilis gluconate operon. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
The nucleotide sequence (742 bp) of the promoter region of the Bacillus subtilis gluconate (gnt) operon is presented. Nuclease Sl mapping revealed the start point of the transcription and suggested that the expression of this operon is probably regulated at the transcriptional level. The sequences of the -35 and -10 regions suggested that RNA polymerase possessing sigma-43 may recognize this structure. The 223 bp fragment containing 100 bp upstream from the transcription start site actually exhibited a promoter activity when cloned in a promoter probe vector of pPL603B. This promoter activity was highly derepressed and although still under catabolite repression. The fragment on a high copy plasmid could titrate a regulator of the gnt operon so that the expression of the operon on the host chromosome also became derepressed. |
| 巻・号 | 14(3) |
| ページ | 1237-52 |
| 公開日 | 1986-2-11 |
| DOI | 10.1093/nar/14.3.1237 |
| PMID | 2419835 |
| PMC | PMC339500 |
| MeSH | Amino Acid Sequence Bacillus subtilis / genetics* Base Sequence Chromosome Mapping DNA Restriction Enzymes DNA, Bacterial / genetics Genes, Bacterial* Genes, Regulator Gluconates / genetics* Promoter Regions, Genetic* RNA, Bacterial / genetics Repetitive Sequences, Nucleic Acid Repressor Proteins / genetics Transcription, Genetic |
| IF | 11.502 |
| 引用数 | 39 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 原核生物(枯草菌) | MBS121 MBS125 |