RRC ID 33594
著者 Fujita Y, Miwa Y, Galinier A, Deutscher J.
タイトル Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr.
ジャーナル Mol Microbiol
Abstract Catabolite repression of various Bacillus subtilis catabolic operons which carry a cis-acting catabolite-responsive element (CRE), such as the gnt operon, is mediated by CcpA, a protein belonging to the GalR-Lacl family of bacterial transcriptional repressors/activators, and the seryl-phosphorylated form of HPr, a phosphocarrier protein of the phosphoenolpyruvate:sugar phosphotransferase system. Footprinting experiments revealed that the purified CcpA protein interacted with P-ser-HPr to cause specific protection of the gnt CRE against DNase I digestion. The specific recognition of the gnt CRE was confirmed by the results of footprinting experiments using mutant gnt CREs carrying one of the following base substitutions within the CRE consensus sequence: G to T at position +149 or C to T at position +154 (+1 is the gnt transcription initiation nucleotide). The two mutant CREs causing a partial relief from catabolite repression were not protected by the CcpA/P-ser-HPr complex in footprinting experiments. Based on these and previous findings, we propose a molecular mechanism underlying catabolite repression in B. subtilis mediated by CcpA and P-ser-HPr.
巻・号 17(5)
ページ 953-60
公開日 1995-9-1
DOI 10.1111/j.1365-2958.1995.mmi_17050953.x
PMID 8596444
MeSH Amino Acid Sequence Bacillus subtilis / genetics* Bacillus subtilis / metabolism* Bacterial Proteins / metabolism Base Sequence Binding Sites DNA Probes DNA, Bacterial / chemistry DNA, Bacterial / metabolism DNA-Binding Proteins / metabolism* Molecular Sequence Data Operon* Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism* Phosphorylation Phosphoserine Promoter Regions, Genetic Recombinant Proteins / metabolism Repressor Proteins / metabolism* Restriction Mapping
IF 3.418
引用数 182
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(枯草菌) MBS160 MBS161 MBS162