RRC ID |
33595
|
著者 |
Miwa Y, Nagura K, Eguchi S, Fukuda H, Deutscher J, Fujita Y.
|
タイトル |
Catabolite repression of the Bacillus subtilis gnt operon exerted by two catabolite-responsive elements.
|
ジャーナル |
Mol Microbiol
|
Abstract |
Catabolite repression of Bacillus subtilis catabolic operons is supposed to occur via a negative regulatory mechanism involving the recognition of a cis-acting catabolite-responsive element (cre) by a complex of CcpA, which is a member of the GalR-Lacl family of bacterial regulatory proteins, and the seryl-phosphorylated form of HPr (P-ser-HPr), as verified by recent studies on catabolite repression of the gnt operon. Analysis of the gnt promoter region by deletions and point mutations revealed that in addition to the cre in the first gene (gntR) of the gnt operon (credown), this operon contains another cre located in the promoter region (creup). A translational gntR'-'lacZ fusion expressed under the control of various combinations of wild-type and mutant credown and creup was integrated into the chromosomal amyE locus, and then catabolite repression of beta-galactosidase synthesis in the resultant integrants was examined. The in vivo results implied that catabolite repression exerted by creup was probably independent of catabolite repression exerted by credown; both creup and credown catabolite repression involved CcpA. Catabolite repression exerted by creup was independent of P-ser-HPr, and catabolite repression exerted by credown was partially independent of P-ser-HPr. DNase I footprinting experiments indicated that a complex of CcpA and P-ser-HPr did not recognize creup, in contrast to its specific recognition of credown. However, CcpA complexed with glucose-6-phosphate specifically recognized creup as well as credown, but the physiological significance of this complexing is unknown.
|
巻・号 |
23(6)
|
ページ |
1203-13
|
公開日 |
1997-3-1
|
DOI |
10.1046/j.1365-2958.1997.2921662.x
|
PMID |
9106211
|
MeSH |
Bacillus subtilis / genetics
Bacillus subtilis / metabolism*
Bacterial Proteins*
Base Sequence
Cyclic AMP Receptor Protein / genetics
Cyclic AMP Receptor Protein / pharmacology*
DNA Mutational Analysis
DNA, Recombinant / genetics
DNA, Recombinant / physiology
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Enhancer Elements, Genetic*
Gene Expression Regulation, Bacterial / genetics
Gene Expression Regulation, Bacterial / physiology*
Genes, Bacterial / genetics
Genes, Bacterial / physiology*
Glucose-6-Phosphate / genetics
Glucose-6-Phosphate / metabolism
Molecular Sequence Data
Operon / genetics*
Promoter Regions, Genetic / genetics
Promoter Regions, Genetic / physiology
Repressor Proteins / genetics
Repressor Proteins / metabolism
|
IF |
3.418
|
引用数 |
68
|
WOS 分野
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
原核生物(枯草菌) |
MBS138
MBS139
MBS143
MBS144
MBS156
MBS157
MBS158
MBS159
MBS172
MBS173
MBS174
MBS175 |