RRC ID 33726
著者 Habib AG, Masuda K, Yukawa M, Tsuchiya E, Ueno M.
タイトル Long G2 accumulates recombination intermediates and disturbs chromosome segregation at dysfunction telomere in Schizosaccharomyces pombe.
ジャーナル Biochem Biophys Res Commun
Abstract Protection of telomere (Pot1) is a single-stranded telomere binding protein which is essential for chromosome ends protection. Fission yeast Rqh1 is a member of RecQ helicases family which has essential roles in the maintenance of genomic stability and regulation of homologous recombination. Double mutant between fission yeast pot1Δ and rqh1 helicase dead (rqh1-hd) maintains telomere by homologous recombination. In pot1Δ rqh1-hd double mutant, recombination intermediates accumulate near telomere which disturb chromosome segregation and make cells sensitive to microtubule inhibitors thiabendazole (TBZ). Deletion of chk1(+) or mutation of its kinase domain shortens the G2 of pot1Δ rqh1-hd double mutant and suppresses both the accumulation of recombination intermediates and the TBZ sensitivity of that double mutant. In this study, we asked whether the long G2 is the reason for the TBZ sensitivity of pot1Δ rqh1-hd double mutant. We found that shortening the G2 of pot1Δ rqh1-hd double mutant by additional mutations of wee1 and mik1 or gain of function mutation of Cdc2 suppresses both the accumulation of recombination intermediates and the TBZ sensitivity of pot1Δ rqh1-hd double mutant. Our results suggest that long G2 of pot1Δ rqh1-hd double mutant may allow time for the accumulation of recombination intermediates which disturb chromosome segregation and make cells sensitive to TBZ.
巻・号 464(1)
ページ 140-6
公開日 2015-8-14
DOI 10.1016/j.bbrc.2015.06.098
PII S0006-291X(15)30150-9
PMID 26093291
MeSH Binding Sites CDC2 Protein Kinase / genetics CDC2 Protein Kinase / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Checkpoint Kinase 1 Chromosome Segregation / drug effects* Chromosomes, Fungal / chemistry Chromosomes, Fungal / drug effects Chromosomes, Fungal / metabolism DNA Helicases / genetics DNA Helicases / metabolism G2 Phase / drug effects G2 Phase / genetics* Gene Expression Regulation, Fungal* Genome, Fungal* Genomic Instability Homologous Recombination / drug effects* Microbial Sensitivity Tests Microtubules / chemistry Microtubules / drug effects Microtubules / metabolism Mutation Nuclear Proteins / genetics Nuclear Proteins / metabolism Protein Binding Protein Kinases / deficiency Protein Kinases / genetics Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism Schizosaccharomyces / drug effects Schizosaccharomyces / genetics* Schizosaccharomyces / growth & development Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Shelterin Complex Telomere / chemistry Telomere / drug effects Telomere / metabolism* Telomere-Binding Proteins / genetics Telomere-Binding Proteins / metabolism Thiabendazole / pharmacology Tubulin Modulators / pharmacology
IF 2.985
引用数 0
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS
リソース情報
酵母 FY16194 MY444