| RRC ID |
34557
|
| 著者 |
Sutani T, Sakata T, Nakato R, Masuda K, Ishibashi M, Yamashita D, Suzuki Y, Hirano T, Bando M, Shirahige K.
|
| タイトル |
Condensin targets and reduces unwound DNA structures associated with transcription in mitotic chromosome condensation.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Chromosome condensation is a hallmark of mitosis in eukaryotes and is a prerequisite for faithful segregation of genetic material to daughter cells. Here we show that condensin, which is essential for assembling condensed chromosomes, helps to preclude the detrimental effects of gene transcription on mitotic condensation. ChIP-seq profiling reveals that the fission yeast condensin preferentially binds to active protein-coding genes in a transcription-dependent manner during mitosis. Pharmacological and genetic attenuation of transcription largely rescue bulk chromosome segregation defects observed in condensin mutants. We also demonstrate that condensin is associated with and reduces unwound DNA segments generated by transcription, providing a direct link between an in vitro activity of condensin and its in vivo function. The human condensin isoform condensin I also binds to unwound DNA regions at the transcription start sites of active genes, implying that our findings uncover a fundamental feature of condensin complexes.
|
| 巻・号 |
6
|
| ページ |
7815
|
| 公開日 |
2015-7-23
|
| DOI |
10.1038/ncomms8815
|
| PII |
ncomms8815
|
| PMID |
26204128
|
| PMC |
PMC4525155
|
| MeSH |
Adenosine Triphosphatases / metabolism*
Chromosomes / metabolism*
DNA-Binding Proteins / metabolism*
Genome
HeLa Cells
Humans
Mitosis
Multiprotein Complexes / metabolism*
RNA Polymerase II / metabolism
Schizosaccharomyces / metabolism*
Transcription, Genetic*
|
| IF |
12.121
|
| 引用数 |
49
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| 酵母 |
FY11487
FY7946
FY8026
FY8027
FY19976 |
