| RRC ID |
34755
|
| 著者 |
Fan J, Kodama E, Koh Y, Nakao M, Matsuoka M.
|
| タイトル |
Halogenated thymidine analogues restore the expression of silenced genes without demethylation.
|
| ジャーナル |
Cancer Res
|
| Abstract |
Transcriptional silencing of tumor suppressor genes by aberrant DNA methylation is a characteristic frequently observed in cancer cells. Therefore, reversing this process is a therapeutic target against cancer. In this study, we established a screening system for silencing inhibitors with cell lines transfected by a retroviral vector containing a luciferase gene. More than 100 nucleosides were tested for antisilencing activity with a selected clone in which the silenced expression of luciferase could be recovered by 5-aza-2'-deoxycytidine. A group of halogenated thymidine analogues was found to reactivate transcription of not only the reporter retrovirus vector but also endogenous glutathione-S-transferase 1 gene, without influence to DNA hypermethylation. Gel mobility shift assay showed that 5-bromo-2'-deoxyuridine (BrdUrd) or 5-iodo-2'-deoxyuridine incorporation did not affect the binding of the methyl-CpG binding protein motif to methylated DNA. Finally, in the retroviral promoter, BrdUrd treatment increased the acetylated histone H3 level and decreased methylation of histone H3 Lys9 in accordance with recovered transcription. This study shows that halogenated thymidines have an antisilencing effect without changing DNA methylation status by interfering with step(s) between DNA methylation and histone acetylation.
|
| 巻・号 |
65(15)
|
| ページ |
6927-33
|
| 公開日 |
2005-8-1
|
| DOI |
10.1158/0008-5472.CAN-04-3495
|
| PII |
65/15/6927
|
| PMID |
16061677
|
| MeSH |
Antimetabolites, Antineoplastic / pharmacology
Base Sequence
Bromodeoxyuridine / pharmacology
Cell Line, Tumor
CpG Islands / drug effects
CpG Islands / genetics
DNA / drug effects
DNA / genetics
DNA / metabolism
DNA Methylation / drug effects
Drug Screening Assays, Antitumor / methods
Gene Expression Regulation, Neoplastic / drug effects
Gene Silencing / drug effects*
Genetic Vectors / genetics
Histones / drug effects
Histones / genetics
Humans
Hydrocarbons, Halogenated / pharmacology
K562 Cells
Molecular Sequence Data
Moloney murine leukemia virus / drug effects
Moloney murine leukemia virus / genetics
Thymidine / analogs & derivatives*
Thymidine / pharmacology*
|
| IF |
9.727
|
| 引用数 |
10
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pCMV-VSV-G (RDB04392) |
