RRC ID 34780
著者 Yoshida S, Higuchi M, Shoji T, Yoshita M, Ishioka K, Takahashi M, Oie M, Tanaka Y, Uchiyama M, Fujii M.
タイトル Knockdown of synapse-associated protein Dlg1 reduces syncytium formation induced by human T-cell leukemia virus type 1.
ジャーナル Virus Genes
Abstract Human T-cell leukemia virus type 1 (HTLV-1) spreads through cell-to-cell contact by forming a virological synapse. Based on the finding that HTLV-1 envelope glycoprotein (Env) binds to a PDZ domain containing scaffold protein Dlg1, whose function has been implicated in the organization of neuronal and immunological synapses, we examined the role of Dlg1 in the cell-cell infection by HTLV-1. The coculture of an HTLV-1-infected T-cell line MT-2 with an uninfected MOLT-4 induced syncytium, a marker of cell-cell HTLV-1 infection, but an RNA interference-mediated knockdown of Dlg1 in both cells cooperatively reduced the syncytium formation. In HTLV-1-uninfected 293T cells, Dlg1 induced the clustering of GLUT1, a cellular receptor for HTLV-1, but such clustering was abrogated by a deletion of the PDZ domain binding motif of GLUT1 (GLUT1DeltaC). GLUT1 expression in MDBK cells induced HTLV-1-mediated syncytium formation, and the activity was much greater than that of GLUT1DeltaC. These results suggest that Dlg1, through the interaction with GLUT1 as well as Env, plays a positive role in the syncytium formation induced by HTLV-1.
巻・号 37(1)
ページ 9-15
公開日 2008-8-1
DOI 10.1007/s11262-008-0234-0
PMID 18461433
MeSH Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Cattle Cell Communication* Cell Fusion Cell Line Discs Large Homolog 1 Protein Gene Expression Giant Cells / metabolism Giant Cells / virology* Glucose Transporter Type 1 / chemistry Glucose Transporter Type 1 / genetics Glucose Transporter Type 1 / metabolism HTLV-I Infections / metabolism HTLV-I Infections / physiopathology* HTLV-I Infections / virology Human T-lymphotropic virus 1 / physiology* Humans Membrane Proteins / genetics Membrane Proteins / metabolism* PDZ Domains RNA Interference* Receptors, Virus / chemistry Receptors, Virus / genetics Receptors, Virus / metabolism Sequence Deletion T-Lymphocytes / physiology* T-Lymphocytes / virology
IF 1.991
引用数 11
WOS 分野 GENETICS & HEREDITY VIROLOGY
リソース情報
遺伝子材料 CS-CDF-CG-PRE (RDB04379) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393)
ヒト・動物細胞 MDCK(RCB0995)