RRC ID |
34780
|
著者 |
Yoshida S, Higuchi M, Shoji T, Yoshita M, Ishioka K, Takahashi M, Oie M, Tanaka Y, Uchiyama M, Fujii M.
|
タイトル |
Knockdown of synapse-associated protein Dlg1 reduces syncytium formation induced by human T-cell leukemia virus type 1.
|
ジャーナル |
Virus Genes
|
Abstract |
Human T-cell leukemia virus type 1 (HTLV-1) spreads through cell-to-cell contact by forming a virological synapse. Based on the finding that HTLV-1 envelope glycoprotein (Env) binds to a PDZ domain containing scaffold protein Dlg1, whose function has been implicated in the organization of neuronal and immunological synapses, we examined the role of Dlg1 in the cell-cell infection by HTLV-1. The coculture of an HTLV-1-infected T-cell line MT-2 with an uninfected MOLT-4 induced syncytium, a marker of cell-cell HTLV-1 infection, but an RNA interference-mediated knockdown of Dlg1 in both cells cooperatively reduced the syncytium formation. In HTLV-1-uninfected 293T cells, Dlg1 induced the clustering of GLUT1, a cellular receptor for HTLV-1, but such clustering was abrogated by a deletion of the PDZ domain binding motif of GLUT1 (GLUT1DeltaC). GLUT1 expression in MDBK cells induced HTLV-1-mediated syncytium formation, and the activity was much greater than that of GLUT1DeltaC. These results suggest that Dlg1, through the interaction with GLUT1 as well as Env, plays a positive role in the syncytium formation induced by HTLV-1.
|
巻・号 |
37(1)
|
ページ |
9-15
|
公開日 |
2008-8-1
|
DOI |
10.1007/s11262-008-0234-0
|
PMID |
18461433
|
MeSH |
Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Animals
Cattle
Cell Communication*
Cell Fusion
Cell Line
Discs Large Homolog 1 Protein
Gene Expression
Giant Cells / metabolism
Giant Cells / virology*
Glucose Transporter Type 1 / chemistry
Glucose Transporter Type 1 / genetics
Glucose Transporter Type 1 / metabolism
HTLV-I Infections / metabolism
HTLV-I Infections / physiopathology*
HTLV-I Infections / virology
Human T-lymphotropic virus 1 / physiology*
Humans
Membrane Proteins / genetics
Membrane Proteins / metabolism*
PDZ Domains
RNA Interference*
Receptors, Virus / chemistry
Receptors, Virus / genetics
Receptors, Virus / metabolism
Sequence Deletion
T-Lymphocytes / physiology*
T-Lymphocytes / virology
|
IF |
1.991
|
引用数 |
11
|
WOS 分野
|
GENETICS & HEREDITY
VIROLOGY
|
リソース情報 |
遺伝子材料 |
CS-CDF-CG-PRE (RDB04379)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393) |
ヒト・動物細胞 |
MDCK(RCB0995) |