RRC ID 34787
著者 Kobayashi K, Takahashi H, Inoue A, Harada H, Toshimori S, Kobayashi Y, Goto K, Sugimoto K, Yano H, Ohnishi T, Tanaka J.
タイトル Oct-3/4 promotes migration and invasion of glioblastoma cells.
ジャーナル J Cell Biochem
Abstract As a result of increased glioblastoma migration and invasion into normal brain parenchyma, treatment of local tumor recurrence following initial treatment in glioblastoma patients remains challenging. Recent studies have demonstrated increased Oct-3/4 expression, a self-renewal regulator in stem cells, in glioblastomas. However, little is known regarding the influence of Oct-3/4 in glioblastoma cell invasiveness. The present study established Oct-3/4-overexpressing glioblastoma cells, which were prepared from human glioblastoma patients, to assess migration, invasion, and mRNA expression profiles of integrins and matrix metalloproteinases (MMPs). Compared with control cells, Oct-3/4 expressing-glioblastoma cells exhibited increased migration and invasion in wound healing and Matrigel invasion assays. Oct-3/4 overexpression resulted in upregulated FAK and c-Src expression, which mediate integrin signals. Vinculin accumulated along the leading edges of Oct-3/4 expressing-glioblastoma cells and associated with membrane ruffles during cell migration. Oct-3/4 expressing-cells exhibited increased MMP-13 mRNA expression and MMP-13 knockdown by shRNA suppressed cell invasion into Matrigel and organotypic brain slices. These results suggested that Oct-3/4 enhanced degradation of surrounding extracellular matrix by increasing MMP-13 expression and altering integrin signaling. Therefore, Oct-3/4 might contribute to tumor promoting activity in glioblastomas.
巻・号 113(2)
ページ 508-17
公開日 2012-2-1
DOI 10.1002/jcb.23374
PMID 21938739
MeSH Actin Cytoskeleton / metabolism Animals Brain / pathology Brain Neoplasms / metabolism Brain Neoplasms / pathology* Cell Movement* Focal Adhesions / metabolism Gene Expression Gene Expression Regulation, Neoplastic Glioblastoma / metabolism Glioblastoma / pathology* Humans Integrins / genetics Integrins / metabolism Matrix Metalloproteinase 13 / genetics Matrix Metalloproteinase 13 / metabolism Neoplasm Invasiveness Octamer Transcription Factor-3 / genetics Octamer Transcription Factor-3 / metabolism* Rats Rats, Wistar Tissue Culture Techniques Tumor Cells, Cultured Up-Regulation
IF 4.237
引用数 28
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
遺伝子材料 pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393)