RRC ID 3481
著者 Zhang P, Aso Y, Jikuya H, Kusakabe T, Lee JM, Kawaguchi Y, Yamamoto K, Banno Y, Fujii H.
タイトル Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis.
ジャーナル J Proteome Res
Abstract The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.
巻・号 6(6)
ページ 2295-303
公開日 2007-6-1
DOI 10.1021/pr070071y
PMID 17497908
MeSH Animals Bombyx / growth & development* Bombyx / metabolism Electrophoresis, Gel, Two-Dimensional Larva / chemistry Metamorphosis, Biological* Muscle Proteins / analysis* Muscle, Skeletal / chemistry* Muscle, Skeletal / growth & development Protein Isoforms / analysis Proteomics* Pupa / chemistry
IF 4.074
引用数 18
WOS 分野 BIOCHEMICAL RESEARCH METHODS
リソース情報
カイコ p22