RRC ID 34833
著者 Ota H, Hikita T, Sawada M, Nishioka T, Matsumoto M, Komura M, Ohno A, Kamiya Y, Miyamoto T, Asai N, Enomoto A, Takahashi M, Kaibuchi K, Sobue K, Sawamoto K.
タイトル Speed control for neuronal migration in the postnatal brain by Gmip-mediated local inactivation of RhoA.
ジャーナル Nat Commun
Abstract Throughout life, new neurons generated in the ventricular-subventricular zone take the long journey to the olfactory bulb. The intracellular mechanisms that precisely control the neurons' migration speed, enabling their well-organized movement, remain unclear. Rho signalling is known to affect the morphology and movement of various cell types, including neurons. Here we identify Gem-interacting protein (Gmip), a RhoA-specific GTPase-activating protein, as a key factor in saltatory neuronal migration. RhoA is activated at the proximal leading process of migrating neurons, where Gmip is also localized and negatively regulates RhoA. Gmip controls the saltatory movement of neurons that regulate their migration speed and 'stop' positions in the olfactory bulb, thereby altering the neural circuitry. This study demonstrates that Gmip serves as a brake for the RhoA-mediated movement of neuronal somata, and highlights the significance of speed control in the well-organized neuronal migration and the maintenance of neuronal circuits in the postnatal brain.
巻・号 5
ページ 4532
公開日 2014-7-30
DOI 10.1038/ncomms5532
PII ncomms5532
PMID 25074242
MeSH Animals Blotting, Western Brain / cytology* Brain / growth & development* Bromodeoxyuridine Cell Movement / physiology* GTPase-Activating Proteins / metabolism* Immunohistochemistry Immunoprecipitation Mass Spectrometry Mice Mice, Knockout Microfilament Proteins / metabolism Microscopy, Confocal Neurons / physiology* RNA Interference Rats Rats, Wistar Signal Transduction / physiology* Statistics, Nonparametric Time-Lapse Imaging Vesicular Transport Proteins / metabolism rhoA GTP-Binding Protein / metabolism*
IF 12.121
引用数 24
WOS 分野 NEUROSCIENCES
リソース情報
遺伝子材料 pEGFPN1/Gmip (RDB13127) pEGFPN1/Gmip delta GAP (RDB13128) pEGFPN1/Gmip delta GAP and CRR (RDB13129).