| RRC ID |
3487
|
| 著者 |
Fujii T, Yokoyama T, Ninagi O, Kakehashi K, Obara Y, Nenoi M, Ishikawa T, Mita K, Shimada T, Abe H.
|
| タイトル |
Isolation and characterization of sex chromosome rearrangements generating male muscle dystrophy and female abnormal oogenesis in the silkworm, Bombyx mori.
|
| ジャーナル |
Genetica
|
| Abstract |
In deletion-mapping of W-specific RAPD (W-RAPD) markers and putative female determinant gene (Fem), we used X-ray irradiation to break the translocation-carrying W chromosome (W( Ze )). We succeeded in obtaining a fragment of the W( Ze ) chromosome designated as Ze (W), having 3 of 12 W-RAPD markers (W-Bonsai, W-Yukemuri-S, W-Yukemuri-L). Inheritance of the Ze (W) fragment by males indicates that it does not include the Fem gene. On the basis of these results, we determined the relative positions of W-Yukemuri-S and W-Yukemuri-L, and we narrowed down the region where Fem gene is located. In addition to the Ze (W) fragment, the Z chromosome was also broken into a large fragment (Z(1)) having the +( sch ) (1-21.5) and a small fragment (Z(2)) having the +( od ) (1-49.6). Moreover, a new chromosomal fragment (Ze (W)Z(2)) was generated by a fusion event between the Ze (W) and the Z(2) fragments. We analyzed the genetic behavior of the Z(1) fragment and the Ze (W)Z(2) fragment during male (Z/Z(1) Ze (W)Z(2)) and female (Z(1) Ze (W)Z(2)/W) meiosis using phenotypic markers. It was observed that the Z(1) fragment and the Z or the W chromosomes separate without fail. On the other hand, non-disjunction between the Ze (W)Z(2) fragment and the Z chromosome and also between the Ze (W)Z(2) fragment and the W chromosome occurred. Furthermore, the females (2A: Z/Ze (W)Z(2)/W) and males (2A: Z/Z(1)) resulting from non-disjunction between the Ze (W)Z(2) fragment and the W chromosome had phenotypic defects: namely, females exhibited abnormal oogenesis and males were flapless due to abnormal indirect flight muscle structure. These results suggest that Z(2) region of the Z chromosome contains dose-sensitive gene(s), which are involved in oogenesis and indirect flight muscle development.
|
| 巻・号 |
130(3)
|
| ページ |
267-80
|
| 公開日 |
2007-7-1
|
| DOI |
10.1007/s10709-006-9104-7
|
| PMID |
17031495
|
| MeSH |
Animals
Bombyx / genetics*
Chromosome Aberrations*
Chromosome Mapping
Female
Gene Deletion
Genetic Markers
Male
Models, Genetic
Muscular Dystrophies / genetics
Oogenesis
Sex Chromosomes*
Sex Factors
Translocation, Genetic
|
| IF |
1.186
|
| 引用数 |
6
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| カイコ |
p50 |
