論文 - 詳細
RRC ID | 35132 |
---|---|
著者 | Sangwallek J, Kaneko Y, Sugiyama M, Ono H, Bamba T, Fukusaki E, Harashima S. |
タイトル | Ketoacyl synthase domain is a major determinant for fatty acyl chain length in Saccharomyces cerevisiae. |
ジャーナル | Arch Microbiol |
Abstract |
Yeast fatty acid synthase (Fas) comprises two subunits, α6 and β6, encoded by FAS2 and FAS1, respectively. To determine features of yeast Fas that control fatty acyl chain length, chimeric genes were constructed by combining FAS sequences from Saccharomyces cerevisiae (ScFAS) and Hansenula polymorpha (HpFAS), which mostly produces C16 and C18 fatty acids, respectively. The C16/C18 ratios decreased from 2.2 ± 0.1 in wild-type S. cerevisiae to 1.0 ± 0.1, 0.5 ± 0.2 and 0.8 ± 0.1 by replacement of ScFAS1, ScFAS2 and ScFAS1 ScFAS2 with HpFAS1, HpFAS2 and HpFAS1 HpFAS2, respectively, suggesting that the α, but not β subunits play a major role in determining fatty acyl chain length. Replacement of phosphopantetheinyl transferase (PPT) domain with the equivalent region from HpFAS2 did not affect C16/C18 ratio. Chimeric Fas2 containing half N-terminal ScFas2 and half C-terminal HpFas2 carrying H. polymorpha ketoacyl synthase (KS) and PPT gave a remarkable decrease in C16/C18 ratio (0.6 ± 0.1), indicating that KS plays a major role in determining chain length. |
巻・号 | 195(12) |
ページ | 843-52 |
公開日 | 2013-12-1 |
DOI | 10.1007/s00203-013-0933-3 |
PMID | 24201996 |
MeSH | Anti-Infective Agents, Local / pharmacology Base Sequence Ethanol / pharmacology Fatty Acid Synthases / genetics Fatty Acids / chemistry* Fatty Acids / genetics Hot Temperature Pichia / enzymology Pichia / genetics Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / enzymology* Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / growth & development Saccharomyces cerevisiae Proteins / genetics |
IF | 1.884 |
引用数 | 4 |
WOS 分野 | MICROBIOLOGY |
リソース情報 | |
酵母 | BY8938(SH8938) BY8940(SH8940) BY8942(SH8942) BY8943(SH8943) BY8944(SH8944) BY4848(SH4848 (W303-1A)) BY19993(KYC1289) BY19994(KYC1290) BY19995(KYC1291) BY19996(KYC1292) BY19997(KYC1293) BY19998(KYC1294) BY8948(SH8948) BY8953(SH8953) BY8958(SH8958) BY19501(H50-5C) |