RRC ID |
35234
|
著者 |
Seshadri A, Dubey B, Weber MH, Varshney U.
|
タイトル |
Impact of rRNA methylations on ribosome recycling and fidelity of initiation in Escherichia coli.
|
ジャーナル |
Mol Microbiol
|
Abstract |
Ribosomal RNA (rRNA) contains a number of modified nucleosides in functionally important regions including the intersubunit bridge regions. As the activity of ribosome recycling factor (RRF) in separating the large and the small subunits of the ribosome involves disruption of intersubunit bridges, we investigated the impact of rRNA methylations on ribosome recycling. We show that deficiency of rRNA methylations, especially at positions 1518 and 1519 of 16S rRNA near the interface with the 50S subunit and in the vicinity of the IF3 binding site, adversely affects the efficiency of RRF-mediated ribosome recycling. In addition, we show that a compromise in the RRF activity affords increased initiation with a mutant tRNA(fMet) wherein the three consecutive G-C base pairs ((29)GGG(31):(39)CCC(41)), a highly conserved feature of the initiator tRNAs, were mutated to those found in the elongator tRNA(Met) ((29)UCA(31):(39)ψGA(41)). This observation has allowed us to uncover a new role of RRF as a factor that contributes to fidelity of initiator tRNA selection on the ribosome. We discuss these and earlier findings to propose that RRF plays a crucial role during all the steps of protein synthesis.
|
巻・号 |
72(3)
|
ページ |
795-808
|
公開日 |
2009-5-1
|
DOI |
10.1111/j.1365-2958.2009.06685.x
|
PII |
MMI6685
|
PMID |
19400784
|
MeSH |
Escherichia coli / genetics
Escherichia coli / metabolism*
Methylation
Mutation
Protein Biosynthesis
RNA, Bacterial / chemistry*
RNA, Ribosomal / chemistry*
RNA, Transfer, Met / metabolism
Ribosomal Proteins / metabolism
Ribosomes / chemistry*
|
IF |
3.418
|
引用数 |
25
|
WOS 分野
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
JW4333-KC(rsmC)? |