RRC ID 35234
著者 Seshadri A, Dubey B, Weber MH, Varshney U.
タイトル Impact of rRNA methylations on ribosome recycling and fidelity of initiation in Escherichia coli.
ジャーナル Mol Microbiol
Abstract Ribosomal RNA (rRNA) contains a number of modified nucleosides in functionally important regions including the intersubunit bridge regions. As the activity of ribosome recycling factor (RRF) in separating the large and the small subunits of the ribosome involves disruption of intersubunit bridges, we investigated the impact of rRNA methylations on ribosome recycling. We show that deficiency of rRNA methylations, especially at positions 1518 and 1519 of 16S rRNA near the interface with the 50S subunit and in the vicinity of the IF3 binding site, adversely affects the efficiency of RRF-mediated ribosome recycling. In addition, we show that a compromise in the RRF activity affords increased initiation with a mutant tRNA(fMet) wherein the three consecutive G-C base pairs ((29)GGG(31):(39)CCC(41)), a highly conserved feature of the initiator tRNAs, were mutated to those found in the elongator tRNA(Met) ((29)UCA(31):(39)ψGA(41)). This observation has allowed us to uncover a new role of RRF as a factor that contributes to fidelity of initiator tRNA selection on the ribosome. We discuss these and earlier findings to propose that RRF plays a crucial role during all the steps of protein synthesis.
巻・号 72(3)
ページ 795-808
公開日 2009-5-1
DOI 10.1111/j.1365-2958.2009.06685.x
PII MMI6685
PMID 19400784
MeSH Escherichia coli / genetics Escherichia coli / metabolism* Methylation Mutation Protein Biosynthesis RNA, Bacterial / chemistry* RNA, Ribosomal / chemistry* RNA, Transfer, Met / metabolism Ribosomal Proteins / metabolism Ribosomes / chemistry*
IF 3.418
引用数 25
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) JW4333-KC(rsmC)?