| RRC ID |
35249
|
| 著者 |
Castro ME, Molina RC, Díaz WA, Pradenas GA, Vásquez CC.
|
| タイトル |
Expression of Aeromonas caviae ST pyruvate dehydrogenase complex components mediate tellurite resistance in Escherichia coli.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Potassium tellurite (K(2)TeO(3)) is harmful to most organisms and specific mechanisms explaining its toxicity are not well known to date. We previously reported that the lpdA gene product of the tellurite-resistant environmental isolate Aeromonas caviae ST is involved in the reduction of tellurite to elemental tellurium. In this work, we show that expression of A. caviae ST aceE, aceF, and lpdA genes, encoding pyruvate dehydrogenase, dihydrolipoamide transacetylase, and dihydrolipoamide dehydrogenase, respectively, results in tellurite resistance and decreased levels of tellurite-induced superoxide in Escherichia coli. In addition to oxidative damage resulting from tellurite exposure, a metabolic disorder would be simultaneously established in which the pyruvate dehydrogenase complex would represent an intracellular tellurite target. These results allow us to widen our vision regarding the molecular mechanisms involved in bacterial tellurite resistance by correlating tellurite toxicity and key enzymes of aerobic metabolism.
|
| 巻・号 |
380(1)
|
| ページ |
148-52
|
| 公開日 |
2009-2-27
|
| DOI |
10.1016/j.bbrc.2009.01.078
|
| PII |
S0006-291X(09)00097-7
|
| PMID |
19168030
|
| MeSH |
Aeromonas / enzymology*
Aeromonas / genetics
Drug Resistance, Bacterial / genetics*
Escherichia coli / drug effects
Escherichia coli / genetics
Pyruvate Dehydrogenase Complex / genetics
Pyruvate Dehydrogenase Complex / metabolism*
Tellurium / toxicity*
|
| IF |
2.985
|
| 引用数 |
18
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9062(BW25113)
JW0110
JW0111
JW0112
ME8993(QC774) |
