RRC ID 35289
著者 Sugihara S, Orikasa Y, Okuyama H.
タイトル The Escherichia coli highly expressed entD gene complements the pfaE deficiency in a pfa gene clone responsible for the biosynthesis of long-chain n-3 polyunsaturated fatty acids.
ジャーナル FEMS Microbiol Lett
Abstract The Escherichia coli entD gene, which encodes an Sfp-type phosphopantetheinyl transferase (PPTase) that is involved in the biosynthesis of siderophore, is available as a high-expression ASKA clone (pCA24N::entD) constructed from the E. coli K-12 strain AG1. In E. coli DH5alpha, pCA24N::entD complemented a pfaE-deficient clone that comprised pfaA, pfaB, pfaC and pfaD, which are four of the five pfa genes that are responsible for the biosynthesis of eicosapentaenoic acid derived from Shewanella pneumatophori SCRC-2738. Sfp-type PPTases are classified into the EntD and PfaE groups, based on differences between their N-terminal-domain structures. Here, we showed that all Sfp-type PPTases may have the potential to promote the biosynthesis of long-chain n-3 polyunsaturated fatty acids.
巻・号 307(2)
ページ 207-11
公開日 2010-6-1
DOI 10.1111/j.1574-6968.2010.01987.x
PII FML1987
PMID 20636978
PMC PMC2901515
MeSH Bacterial Proteins / biosynthesis* Bacterial Proteins / genetics Bacterial Proteins / metabolism Chromatography, Gas Cloning, Molecular Electrophoresis, Polyacrylamide Gel Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins / biosynthesis* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Fatty Acids, Unsaturated / metabolism* Genes, Bacterial Genetic Complementation Test Ligases / biosynthesis* Ligases / genetics Ligases / metabolism Plasmids / genetics Transferases (Other Substituted Phosphate Groups) / biosynthesis* Transferases (Other Substituted Phosphate Groups) / genetics Transferases (Other Substituted Phosphate Groups) / metabolism Transformation, Bacterial
IF 1.987
引用数 7
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME5305(AG1) ASKA library