RRC ID 35314
著者 Uehara T, Parzych KR, Dinh T, Bernhardt TG.
タイトル Daughter cell separation is controlled by cytokinetic ring-activated cell wall hydrolysis.
ジャーナル EMBO J
Abstract During bacterial cytokinesis, hydrolytic enzymes are used to split wall material shared by adjacent daughter cells to promote their separation. Precise control over these enzymes is critical to prevent breaches in wall integrity that can cause cell lysis. How these potentially lethal hydrolases are regulated has remained unknown. Here, we investigate the regulation of cell wall turnover at the Escherichia coli division site. We show that two components of the division machinery with LytM domains (EnvC and NlpD) are direct regulators of the cell wall hydrolases (amidases) responsible for cell separation (AmiA, AmiB and AmiC). Using in vitro cell wall cleavage assays, we show that EnvC activates AmiA and AmiB, whereas NlpD activates AmiC. Consistent with these findings, we show that an unregulated EnvC mutant requires functional AmiA or AmiB but not AmiC to induce cell lysis, and that the loss of NlpD phenocopies an AmiC(-) defect. Overall, our results suggest that cellular amidase activity is regulated spatially and temporally by coupling their activation to the assembly of the cytokinetic ring.
巻・号 29(8)
ページ 1412-22
公開日 2010-4-21
DOI 10.1038/emboj.2010.36
PII emboj201036
PMID 20300061
PMC PMC2868575
MeSH Amidohydrolases / metabolism* Cell Wall / enzymology* Cytokinesis* Enzyme Activation Escherichia coli / cytology* Escherichia coli / enzymology* Escherichia coli Proteins / chemistry Escherichia coli Proteins / metabolism Hydrolysis Multidrug Resistance-Associated Proteins / chemistry Multidrug Resistance-Associated Proteins / metabolism Peptidoglycan / metabolism* Protein Structure, Tertiary
IF 9.889
引用数 163
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
原核生物(大腸菌) ME9062(BW25113) JW5646-KC JW2712-KC JW1667-KC