論文 - 詳細
| RRC ID | 35325 |
|---|---|
| 著者 | Krin E, Danchin A, Soutourina O. |
| タイトル | Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli. |
| ジャーナル | BMC Microbiol |
| Abstract |
BACKGROUND:H-NS regulates the acid stress resistance. The present study aimed to characterize the H-NS-dependent cascade governing the acid stress resistance pathways and to define the interplay between the different regulators. RESULTS:We combined mutational, phenotypic and gene expression analyses, to unravel the regulatory hierarchy in acid resistance involving H-NS, RcsB-P/GadE complex, HdfR, CadC, AdiY regulators, and DNA-binding assays to separate direct effects from indirect ones. RcsB-P/GadE regulatory complex, the general direct regulator of glutamate-, arginine- and lysine-dependent acid resistance pathways plays a central role in the regulatory cascade. However, H-NS also directly controls specific regulators of these pathways (e.g. cadC) and genes involved in general stress resistance (hdeAB, hdeD, dps, adiY). Finally, we found that in addition to H-NS and RcsB, a third regulator, HdfR, inversely controls glutamate-dependent acid resistance pathway and motility. CONCLUSIONS:H-NS lies near the top of the hierarchy orchestrating acid response centred on RcsB-P/GadE regulatory complex, the general direct regulator of glutamate-, arginine- and lysine-dependent acid resistance pathways. |
| 巻・号 | 10 |
| ページ | 273 |
| 公開日 | 2010-10-29 |
| DOI | 10.1186/1471-2180-10-273 |
| PII | 1471-2180-10-273 |
| PMID | 21034467 |
| PMC | PMC2984483 |
| MeSH | Acids / metabolism* Escherichia coli / genetics Escherichia coli / physiology* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Fimbriae Proteins / genetics Fimbriae Proteins / metabolism* Gene Expression Regulation, Bacterial* Stress, Physiological |
| IF | 2.989 |
| 引用数 | 35 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | JD21162 JD24946 JD25275 JD26576 JD27509 JW5594-KC JW2366-KC JW3772-KC |