| RRC ID |
35327
|
| 著者 |
Arenas FA, Díaz WA, Leal CA, Pérez-Donoso JM, Imlay JA, Vásquez CC.
|
| タイトル |
The Escherichia coli btuE gene, encodes a glutathione peroxidase that is induced under oxidative stress conditions.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Most aerobic organisms are exposed to oxidative stress. Looking for enzyme activities involved in the bacterial response to this kind of stress, we focused on the btuE-encoded Escherichia coli BtuE, an enzyme that shares homology with the glutathione peroxidase (GPX) family. This work deals with the purification and characterization of the btuE gene product. Purified BtuE decomposes in vitro hydrogen peroxide in a glutathione-dependent manner. BtuE also utilizes preferentially thioredoxin A to decompose hydrogen peroxide as well as cumene-, tert-butyl-, and linoleic acid hydroperoxides, confirming that its active site confers non-specific peroxidase activity. These data suggest that the enzyme may have one or more organic hydroperoxide as its physiological substrate. The btuE gene was induced when cells were exposed to oxidative stress elicitors that included potassium tellurite, menadione and hydrogen peroxide, among others, suggesting that BtuE could participate in the E. coli response to reactive oxygen species. To our knowledge, this is the first report describing a glutathione peroxidase in E. coli.
|
| 巻・号 |
398(4)
|
| ページ |
690-4
|
| 公開日 |
2010-8-6
|
| DOI |
10.1016/j.bbrc.2010.07.002
|
| PII |
S0006-291X(10)01295-7
|
| PMID |
20621065
|
| PMC |
PMC3057470
|
| MeSH |
Escherichia coli / enzymology*
Escherichia coli / genetics
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Glutathione / chemistry
Glutathione / metabolism
Glutathione Peroxidase / chemistry
Glutathione Peroxidase / genetics
Glutathione Peroxidase / metabolism*
Hydrogen Peroxide / chemistry
Hydrogen Peroxide / metabolism
Oxidative Stress*
Periplasmic Binding Proteins / chemistry
Periplasmic Binding Proteins / genetics
Periplasmic Binding Proteins / metabolism*
|
| IF |
2.985
|
| 引用数 |
44
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9062(BW25113)
ASKA collection |
