| RRC ID |
35337
|
| 著者 |
Typas A, Banzhaf M, van den Berg van Saparoea B, Verheul J, Biboy J, Nichols RJ, Zietek M, Beilharz K, Kannenberg K, von Rechenberg M, Breukink E, den Blaauwen T, Gross CA, Vollmer W.
|
| タイトル |
Regulation of peptidoglycan synthesis by outer-membrane proteins.
|
| ジャーナル |
Cell
|
| Abstract |
Growth of the mesh-like peptidoglycan (PG) sacculus located between the bacterial inner and outer membranes (OM) is tightly regulated to ensure cellular integrity, maintain cell shape, and orchestrate division. Cytoskeletal elements direct placement and activity of PG synthases from inside the cell, but precise spatiotemporal control over this process is poorly understood. We demonstrate that PG synthases are also controlled from outside of the sacculus. Two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional PG synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new PG to the sacculus. LpoB shows partial septal localization, and our data suggest that the LpoB-PBP1B complex contributes to OM constriction during cell division. LpoA/LpoB and their PBP-docking regions are restricted to γ-proteobacteria, providing models for niche-specific regulation of sacculus growth.
|
| 巻・号 |
143(7)
|
| ページ |
1097-109
|
| 公開日 |
2010-12-23
|
| DOI |
10.1016/j.cell.2010.11.038
|
| PII |
S0092-8674(10)01360-7
|
| PMID |
21183073
|
| PMC |
PMC3060616
|
| MeSH |
Bacterial Outer Membrane Proteins / chemistry
Bacterial Outer Membrane Proteins / metabolism*
Cell Division
Cell Wall / metabolism
Escherichia coli / cytology*
Escherichia coli / enzymology
Escherichia coli / metabolism*
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / metabolism*
Lipoproteins / chemistry
Lipoproteins / metabolism
Penicillin-Binding Proteins / metabolism
Peptidoglycan / biosynthesis*
Peptidoglycan Glycosyltransferase / metabolism
Protein Interaction Domains and Motifs
|
| IF |
38.637
|
| 引用数 |
206
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9062(BW25113)(Keio)
Keio collection
ASKA collection |
