RRC ID 35360
著者 Sun Y, Fukamachi T, Saito H, Kobayashi H.
タイトル ATP requirement for acidic resistance in Escherichia coli.
ジャーナル J Bacteriol
Abstract ATP participates in many cellular metabolic processes as a major substrate to supply energy. Many systems for acidic resistance (AR) under extremely acidic conditions have been reported, but the role of ATP has not been examined. To clarify whether or not ATP is necessary for the AR in Escherichia coli, the AR of mutants deficient in genes for ATP biosynthesis was investigated in this study. The deletion of purA or purB, each of which encodes enzymes to produce AMP from inosinate (IMP), markedly decreased the AR. The content of ATP in these mutants decreased rapidly at pH 2.5 compared to that of the wild type. The AR was again decreased significantly by the mutation of adk, which encoded an enzyme to produce ADP from AMP. The DNA damage in the purA and purB mutants was higher than that in the wild type. These results demonstrated that metabolic processes that require ATP participate in survival under extremely acidic conditions, and that one such system is the ATP-dependent DNA repair system.
巻・号 193(12)
ページ 3072-7
公開日 2011-6-1
DOI 10.1128/JB.00091-11
PII JB.00091-11
PMID 21478347
PMC PMC3133219
MeSH Acids / pharmacology* Adenosine Triphosphate / metabolism* Arginine / metabolism Arginine / pharmacology DNA Damage DNA, Bacterial / genetics Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial / physiology Genome, Bacterial Glutamic Acid / metabolism Glutamic Acid / pharmacology Homeostasis Hydrogen-Ion Concentration Lysine / metabolism Lysine / pharmacology
IF 3.006
引用数 17
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) JW4135-KC JW2461-KC JW0511-KC JW2541-KC JW2491-KC JW5401-KC JW2651-KC JW4197-KC JW2788-KC IH125 4004(purA) 1084(purB)