RRC ID 35373
著者 Ramstedt M, Nakao R, Wai SN, Uhlin BE, Boily JF.
タイトル Monitoring surface chemical changes in the bacterial cell wall: multivariate analysis of cryo-x-ray photoelectron spectroscopy data.
ジャーナル J Biol Chem
Abstract Gram-negative bacteria can alter the composition of the lipopolysaccharide (LPS) layer of the outer membrane as a response to different growth conditions and external stimuli. These alterations can, for example, promote attachment to surfaces and biofilm formation. The changes occur in the outermost layer of the cell and may consequently influence interactions between bacterial cells and surrounding host tissue, as well as other surfaces. Microscopic analyses, fractionation of bacterial cells, or other traditional microbiological assays have previously been used to study these alterations. These methods can, however, be time consuming and do not always give detailed chemical information about the bacterial cell surface. We here present an analytical method that provides chemical information on the outermost portion of bacterial cells with respect to protein, peptidoglycan, lipid, and polysaccharide content. The method involves cryo-x-ray photoelectron spectroscopy analyses of the outermost portion (within ∼10 nm of the surface) of intact bacterial cells followed by a multivariate curve resolution analysis of carbon spectra. It can be used as a tool for characterizing and monitoring variations in the chemical composition of bacterial cell walls or of isolated outer membrane vesicles, variations that result from e.g. mutations or external stimuli. The method enabled us to predict accurately the alterations in polysaccharide content and surface chemistries of a set of well characterized Escherichia coli LPS mutants. The described approach may moreover be applied to monitor surface chemical composition of other biological samples.
巻・号 286(14)
ページ 12389-96
公開日 2011-4-8
DOI 10.1074/jbc.M110.209536
PII S0021-9258(20)51618-9
PMID 21330374
PMC PMC3069442
MeSH Cell Wall / chemistry* Cell Wall / metabolism* Escherichia coli / chemistry Escherichia coli / metabolism Lipopolysaccharides / chemistry Lipopolysaccharides / genetics Lipopolysaccharides / metabolism Multivariate Analysis* Peptidoglycan / chemistry Peptidoglycan / metabolism Photoelectron Spectroscopy / methods*
IF 4.238
引用数 24
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME9062(BW25113) pNT3(hide) pNTR-S.D.