RRC ID 35413
著者 Ollivierre JN, Sikora JL, Beuning PJ.
タイトル The dimeric SOS mutagenesis protein UmuD is active as a monomer.
ジャーナル J Biol Chem
Abstract The homodimeric umuD gene products play key roles in regulating the cellular response to DNA damage in Escherichia coli. UmuD(2) is composed of 139-amino acid subunits and is up-regulated as part of the SOS response. Subsequently, damage-induced RecA·ssDNA nucleoprotein filaments mediate the slow self-cleavage of the N-terminal 24-amino acid arms yielding UmuD'(2). UmuD(2) and UmuD'(2) make a number of distinct protein-protein contacts that both prevent and facilitate mutagenic translesion synthesis. Wild-type UmuD(2) and UmuD'(2) form exceptionally tight dimers in solution; however, we show that the single amino acid change N41D generates stable, active UmuD and UmuD' monomers that functionally mimic the dimeric wild-type proteins. The UmuD N41D monomer is proficient for cleavage and interacts physically with DNA polymerase IV (DinB) and the β clamp. Furthermore, the N41D variants facilitate UV-induced mutagenesis and promote overall cell viability. Taken together, these observations show that a monomeric form of UmuD retains substantial function in vivo and in vitro.
巻・号 286(5)
ページ 3607-17
公開日 2011-2-4
DOI 10.1074/jbc.M110.167254
PII S0021-9258(20)54065-9
PMID 21118802
PMC PMC3030365
MeSH Amino Acid Substitution Cell Survival DNA-Directed DNA Polymerase / genetics DNA-Directed DNA Polymerase / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Mutagenesis* / radiation effects Mutant Proteins / chemistry Mutant Proteins / metabolism Protein Multimerization* Protein Stability SOS Response, Genetics
IF 4.238
引用数 11
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) JW2860-KC?