論文 - 詳細
| RRC ID | 35441 |
|---|---|
| 著者 | Yamamoto M, Nagao M, Hotta G, Matsumura Y, Matsushima A, Ito Y, Takakura S, Ichiyama S. |
| タイトル | Molecular characterization of IMP-type metallo-β-lactamases among multidrug-resistant Achromobacter xylosoxidans. |
| ジャーナル | J Antimicrob Chemother |
| Abstract |
OBJECTIVES:To analyse the metallo-β-lactamase (MBL) genes and their genetic environments in clinical isolates of Achromobacter xylosoxidans. METHODS:From January 2004 to December 2010, four MBL-producing, multidrug-resistant (MDR) A. xylosoxidans strains were isolated and analysed at a tertiary care university hospital in Japan. Species-level identification was confirmed by 16S ribosomal RNA gene sequencing. Molecular typing was performed using PFGE, the presence of MBL genes was detected using PCR, and integron gene cassettes were examined by cloning and sequence analysis of integron PCR products. The plasmids obtained from individual isolates were analysed based on EcoRI restriction patterns, Southern hybridizations using digoxigenin-labelled probes for bla(IMP-1) and bla(IMP-19) as well as conjugation and transformation experiments. RESULTS:No clonal relationship was found among the four A. xylosoxidans isolates. Three isolates harboured bla(IMP-1) and one isolate harboured bla(IMP-19). These MBL genes were carried on class 1 integrons. Four different class 1 integron gene cassette arrays were found, including orf1-bla(IMP-1)-aacA4, bla(IMP-1)-bla(IMP-1)-nit1/nit2-catB3-bla(IMP-1)-bla(IMP-1), aacA4-bla(IMP-1) and bla(IMP-19). The restriction pattern of the plasmid DNA obtained from each isolate was unique and the hybridization analyses revealed the presence of each MBL gene within a plasmid. Moreover, all of the plasmids carrying an MBL gene could be transformed into Escherichia coli HST08. CONCLUSIONS:This study provides genetic evidence for the existence of IMP-type MBL genes in MDR A. xylosoxidans isolates. Moreover, the present findings provide evidence that A. xylosoxidans can receive IMP-type MBL genes via plasmid-mediated transfer, which contributes to their carbapenem resistance. |
| 巻・号 | 67(9) |
| ページ | 2110-3 |
| 公開日 | 2012-9-1 |
| DOI | 10.1093/jac/dks179 |
| PII | dks179 |
| PMID | 22577105 |
| MeSH | Achromobacter denitrificans / drug effects Achromobacter denitrificans / enzymology* Achromobacter denitrificans / genetics* Achromobacter denitrificans / isolation & purification Anti-Bacterial Agents / pharmacology* Blotting, Southern DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Bacterial / metabolism DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Deoxyribonuclease EcoRI / metabolism Drug Resistance, Multiple, Bacterial* Electrophoresis, Gel, Pulsed-Field Gram-Negative Bacterial Infections / microbiology* Hospitals, University Humans Japan Molecular Typing Plasmids / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Tertiary Care Centers beta-Lactamases / genetics* beta-Lactamases / metabolism* |
| IF | 5.439 |
| 引用数 | 23 |
| WOS 分野 | PHARMACOLOGY & PHARMACY INFECTIOUS DISEASES MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | ME8067 |