論文 - 詳細
| RRC ID | 35442 |
|---|---|
| 著者 | Shimazaki J, Furukawa S, Ogihara H, Morinaga Y. |
| タイトル | L-Tryptophan prevents Escherichia coli biofilm formation and triggers biofilm degradation. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The effect of deletion of trp operon and tna operon on the Escherichia coli biofilm formation was investigated in order to elucidate the role of L-tryptophan metabolism in biofilm formation. trp operon deletion mutants ΔtrpC, ΔtrpD and ΔtrpE deficient in L-tryptophan biosynthesis showed higher biofilm formation. In addition, ΔtnaC with increased L-tryptophan degradation activity showed higher biofilm formation. On the contrary, ΔtnaA deletion mutant which lost L-tryptophan degradation activity showed low biofilm formation. From these results, it was suggested that decrease of intracellular L-tryptophan level induced biofilm formation and increase of L-tryptophan repressed biofilm formation. So the effect of the addition of L-tryptophan to the medium on the E. coli biofilm formation was investigated. L-Tryptophan addition at starting culture decreased biofilm formation and furthermore L-tryptophan addition after 16 h culture induced the degradation of preformed biofilm. From the above results, it was suggested that maintenance of high intracellular L-tryptophan concentration prevents E. coli biofilm formation and elevation of intracellular L-tryptophan concentration triggers degradation of matured biofilm. |
| 巻・号 | 419(4) |
| ページ | 715-8 |
| 公開日 | 2012-3-23 |
| DOI | 10.1016/j.bbrc.2012.02.085 |
| PII | S0006-291X(12)00318-X |
| PMID | 22386992 |
| MeSH | Biofilms / drug effects Biofilms / growth & development* Escherichia coli / drug effects Escherichia coli / genetics Escherichia coli / physiology* Gene Deletion Operon / genetics Operon / physiology* Tryptophan / genetics Tryptophan / metabolism* Tryptophan / pharmacology |
| IF | 2.985 |
| 引用数 | 13 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113) JW3686-KC JW3685-KC JW1252-KC JW1253-KC JW1254-KC JW1255-KC JW1256-KC |