論文 - 詳細
| RRC ID | 35446 |
|---|---|
| 著者 | Doukyu N, Ishikawa K, Watanabe R, Ogino H. |
| タイトル | Improvement in organic solvent tolerance by double disruptions of proV and marR genes in Escherichia coli. |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIMS:To investigate the involvement of osmoprotectant transporters in organic solvent tolerance in Escherichia coli and to construct an E. coli strain with high organic solvent tolerance. METHODS AND RESULTS:The organic solvent tolerance of ΔbetT, ΔproV, ΔproP or ΔputP single-gene knockout mutants of E. coli K-12 strain was examined. Among these mutants, the organic solvent tolerance of the ΔproV mutant remarkably increased compared with that of the parent strain. It has been known that a marR mutation confers tolerance on E. coli to organic solvents. A ΔproV and ΔmarR double-gene mutant was more tolerant to organic solvents than the ΔproV or ΔmarR single-gene mutant. The n-hexane amount accumulated in E. coli cells was examined after incubation in an n-hexane-aqueous medium two-phase system. The intracellular n-hexane level in the ΔproV and ΔmarR double-gene mutant was kept lower than those of the parent strain, ΔproV mutant and ΔmarR mutant. CONCLUSIONS:The organic solvent tolerance level in E. coli highly increased by dual disruption of proV and marR. SIGNIFICANCE AND IMPACT OF THE STUDY:This study suggests a new strategy for increasing the organic solvent tolerance level in E. coli to improve the usability of the whole-cell biocatalysts in two-phase systems employing organic solvents. |
| 巻・号 | 112(3) |
| ページ | 464-74 |
| 公開日 | 2012-3-1 |
| DOI | 10.1111/j.1365-2672.2012.05236.x |
| PMID | 22257006 |
| MeSH | Drug Resistance, Bacterial / genetics Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Gene Knockout Techniques Genes, Bacterial Hexanes / pharmacology* Lipoproteins / metabolism Membrane Transport Proteins / genetics* Membrane Transport Proteins / metabolism Mutation Periplasmic Binding Proteins / genetics* Repressor Proteins / genetics* Solvents / pharmacology* |
| IF | 3.066 |
| 引用数 | 19 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113) JW2652-KC JW2653-KC JW2654-KC JW4072-KC JW1001-KC JW5248-KC pCP20? |