論文 - 詳細
| RRC ID | 35449 |
|---|---|
| 著者 | Ojima Y, Suryadarma P, Tsuchida K, Taya M. |
| タイトル | Accumulation of pyruvate by changing the redox status in Escherichia coli. |
| ジャーナル | Biotechnol Lett |
| Abstract |
Pyruvate was produced from glucose by Escherichia coli BW25113 that contained formate dehydrogenase (FDH) from Mycobacterium vaccae. In aerobic shake-flask culture (K (L) a = 4.9 min(-1)), the recombinant strain produced 6.7 g pyruvate l(-1) after 24 h with 4 g sodium formate l(-1) and a yield of 0.34 g pyruvate g glucose(-1). These values were higher than those of the original strain (0.2 g l(-1) pyruvate and 0.02 g pyruvate g glucose(-1)). Based on the reaction mechanism of FDH, the introduction of FDH into E. coli enhances the accumulation of pyruvate by the regeneration of NADH from NAD(+) since NAD(+) is a shared cosubstrate with the pyruvate dehydrogenase complex, which decarboxylates pyruvate to acetyl-CoA and CO(2). The oxygenation level was enough high to inactivate lactate dehydrogenase, which was of benefit to pyruvate accumulation without lactate as a by-product. |
| 巻・号 | 34(5) |
| ページ | 889-93 |
| 公開日 | 2012-5-1 |
| DOI | 10.1007/s10529-011-0842-y |
| PMID | 22215378 |
| MeSH | Aerobiosis Coenzymes / metabolism Escherichia coli / genetics Escherichia coli / metabolism* Formate Dehydrogenases / genetics Formate Dehydrogenases / metabolism* Glucose / metabolism Mycobacterium / enzymology Mycobacterium / genetics NAD / metabolism Oxidation-Reduction Pyruvic Acid / metabolism* Recombinant Proteins / genetics Recombinant Proteins / metabolism |
| IF | 1.977 |
| 引用数 | 14 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113) JW0855-KC JW2294-KC |