RRC ID 35462
著者 Butala M, Sonjak S, Kamenšek S, Hodošček M, Browning DF, Žgur-Bertok D, Busby SJ.
タイトル Double locking of an Escherichia coli promoter by two repressors prevents premature colicin expression and cell lysis.
ジャーナル Mol Microbiol
Abstract The synthesis of Eschericha coli colicins is lethal to the producing cell and is repressed during normal growth by the LexA transcription factor, which is the master repressor of the SOS system for repair of DNA damage. Following DNA damage, LexA is inactivated and SOS repair genes are induced immediately, but colicin production is delayed and induced only in terminally damaged cells. The cause of this delay is unknown. Here we identify the global transcription repressor, IscR, as being directly responsible for the delay in colicin K expression during the SOS response, and identify the DNA target for IscR at the colicin K operon promoter. Our results suggest that, IscR stabilizes LexA at the cka promoter after DNA damage thus, preventing its cleavage and inactivation, and this cooperation ensures that suicidal colicin K production is switched on only as a last resort. A similar mechanism operates at the regulatory region of other colicins and, hence, we suggest that many promoters that control the expression of 'lethal' genes are double locked.
巻・号 86(1)
ページ 129-39
公開日 2012-10-1
DOI 10.1111/j.1365-2958.2012.08179.x
PMID 22812562
MeSH Bacterial Proteins / metabolism* Bacteriolysis Base Sequence Colicins / biosynthesis* Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli / physiology Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial* Models, Biological Molecular Sequence Data Promoter Regions, Genetic* Protein Binding Serine Endopeptidases / metabolism* Transcription Factors / metabolism*
IF 3.418
引用数 24
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) DH5α? ME9062(BW25113) JW2515-KC JW0824-KC JW2947-KC