RRC ID 35553
著者 Gerosa L, Kochanowski K, Heinemann M, Sauer U.
タイトル Dissecting specific and global transcriptional regulation of bacterial gene expression.
ジャーナル Mol Syst Biol
Abstract Gene expression is regulated by specific transcriptional circuits but also by the global expression machinery as a function of growth. Simultaneous specific and global regulation thus constitutes an additional--but often neglected--layer of complexity in gene expression. Here, we develop an experimental-computational approach to dissect specific and global regulation in the bacterium Escherichia coli. By using fluorescent promoter reporters, we show that global regulation is growth rate dependent not only during steady state but also during dynamic changes in growth rate and can be quantified through two promoter-specific parameters. By applying our approach to arginine biosynthesis, we obtain a quantitative understanding of both specific and global regulation that allows accurate prediction of the temporal response to simultaneous perturbations in arginine availability and growth rate. We thereby uncover two principles of joint regulation: (i) specific regulation by repression dominates the transcriptional response during metabolic steady states, largely repressing the biosynthesis genes even when biosynthesis is required and (ii) global regulation sets the maximum promoter activity that is exploited during the transition between steady states.
巻・号 9
ページ 658
公開日 2013-4-16
DOI 10.1038/msb.2013.14
PII msb201314
PMID 23591774
PMC PMC3658269
MeSH Arginine / genetics Arginine / metabolism* Computer Simulation Escherichia coli K12 / genetics* Escherichia coli K12 / growth & development Escherichia coli K12 / metabolism Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial* Genes, Reporter Green Fluorescent Proteins Kinetics Models, Biological Promoter Regions, Genetic Transcription, Genetic*
IF 8.991
引用数 72
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME9062(BW25113)?