| RRC ID |
35560
|
| 著者 |
Lindemann C, Lupilova N, Müller A, Warscheid B, Meyer HE, Kuhlmann K, Eisenacher M, Leichert LI.
|
| タイトル |
Redox proteomics uncovers peroxynitrite-sensitive proteins that help Escherichia coli to overcome nitrosative stress.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Peroxynitrite is a highly reactive chemical species with antibacterial properties that are synthesized in immune cells. In a proteomic approach, we identified specific target proteins of peroxynitrite-induced modifications in Escherichia coli. Although peroxynitrite caused a fairly indiscriminate nitration of tyrosine residues, reversible modifications of protein thiols were highly specific. We used a quantitative redox proteomic method based on isotope-coded affinity tag chemistry and identified four proteins consistently thiol-modified in cells treated with peroxynitrite as follows: AsnB, FrmA, MaeB, and RidA. All four were required for peroxynitrite stress tolerance in vivo. Three of the identified proteins were modified at highly conserved cysteines, and MaeB and FrmA are known to be directly involved in the oxidative and nitrosative stress response in E. coli. In in vitro studies, we could show that the activity of RidA, a recently discovered enamine/imine deaminase, is regulated in a specific manner by the modification of its single conserved cysteine. Mutation of this cysteine 107 to serine generated a constitutively active protein that was not susceptible to peroxynitrite.
|
| 巻・号 |
288(27)
|
| ページ |
19698-714
|
| 公開日 |
2013-7-5
|
| DOI |
10.1074/jbc.M113.457556
|
| PII |
S0021-9258(20)45686-8
|
| PMID |
23696645
|
| PMC |
PMC3707675
|
| MeSH |
Escherichia coli / chemistry
Escherichia coli / genetics
Escherichia coli / metabolism*
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Oxidation-Reduction
Oxidative Stress / drug effects*
Oxidative Stress / genetics
Peroxynitrous Acid / chemistry
Peroxynitrous Acid / pharmacology*
Proteomics*
|
| IF |
4.238
|
| 引用数 |
17
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
JW0660-KC
JW0347-KC
JW2447-KC
JW5755-KC |
