RRC ID 35585
著者 Travier L, Rendueles O, Ferrières L, Herry JM, Ghigo JM.
タイトル Escherichia coli resistance to nonbiocidal antibiofilm polysaccharides is rare and mediated by multiple mutations leading to surface physicochemical modifications.
ジャーナル Antimicrob Agents Chemother
Abstract Antivirulence strategies targeting bacterial behavior, such as adhesion and biofilm formation, are expected to exert low selective pressure and have been proposed as alternatives to biocidal antibiotic treatments to avoid the rapid occurrence of bacterial resistance. Here, we tested this hypothesis using group 2 capsule polysaccharide (G2cps), a polysaccharidic molecule previously shown to impair bacterium-surface interactions, and we investigated the nature of bacterial resistance to a nonbiocidal antibiofilm strategy. We screened an Escherichia coli mutant library for an increased ability to form biofilm in the presence of G2cps, and we identified several mutants displaying partial but not total resistance to this antibiofilm polysaccharide. Our genetic analysis showed that partial resistance to G2cps results from multiple unrelated mutations leading to modifications in surface physicochemical properties that counteract the changes in ionic charge and Lewis base properties induced by G2cps. Moreover, some of the identified mutants harboring improved biofilm formation in the presence of G2cps were also partially resistant to other antibiofilm molecules. This study therefore shows that alterations of bacterial surface properties mediate only partial resistance to G2cps. It also experimentally validates the potential value of nonbiocidal antibiofilm strategies, since full resistance to antibiofilm compounds is rare and potentially unlikely to arise in clinical settings.
巻・号 57(8)
ページ 3960-8
公開日 2013-8-1
DOI 10.1128/AAC.02606-12
PII AAC.02606-12
PMID 23733462
PMC PMC3719787
MeSH Anti-Bacterial Agents / pharmacology Bacterial Adhesion / drug effects Biofilms / drug effects* Chemical Phenomena DNA Transposable Elements Drug Resistance, Multiple, Bacterial Escherichia coli / chemistry Escherichia coli / drug effects* Escherichia coli / genetics Genes, Bacterial* Lewis Bases / chemistry Microbial Sensitivity Tests Mutation* Polymyxin B / pharmacology Polysaccharides / pharmacology* Polysorbates / pharmacology
IF 4.904
引用数 8
WOS 分野 PHARMACOLOGY & PHARMACY MICROBIOLOGY
リソース情報
原核生物(大腸菌) JW5768-KC JW1875-KC JW1411-KC JW0886-KC JW2913-KC JW0784-KC JW5137-KC JW4066-KC