論文 - 詳細
| RRC ID | 35626 |
|---|---|
| 著者 | Kozmin SG, Stepchenkova EI, Chow SC, Schaaper RM. |
| タイトル | A critical role for the putative NCS2 nucleobase permease YjcD in the sensitivity of Escherichia coli to cytotoxic and mutagenic purine analogs. |
| ジャーナル | mBio |
| Abstract |
UNLABELLED:The base analogs 6-N-hydroxylaminopurine (HAP) and 2-amino-HAP (AHAP) are potent mutagens in bacteria and eukaryotic organisms. Previously, we demonstrated that a defect in the Escherichia coli ycbX gene, encoding a molybdenum cofactor-dependent oxidoreductase, dramatically enhances sensitivity to the toxic and mutagenic action of these agents. In the present study, we describe the discovery and properties of a novel suppressor locus, yjcD, that strongly reduces the HAP sensitivity of the ycbX strain. Suppressor effects are also observed for other purine analogs, like AHAP, 6-mercaptopurine, 6-thioguanine, and 2-aminopurine. In contrast, the yjcD defect did not affect the sensitivity to the pyrimidine analog 5-fluorouracil. Homology searches have predicted that yjcD encodes a putative permease of the NCS2 family of nucleobase transporters. We further investigated the effects of inactivation of all other members of the NCS2 family, XanQ, XanP, PurP, UacT, UraA, RutG, YgfQ, YicO, and YbbY, and of the NCS1 family nucleobase permeases CodB and YbbW. None of these other defects significantly affected sensitivity to either HAP or AHAP. The combined data strongly suggest that YjcD is the primary importer for modified purine bases. We also present data showing that this protein may, in fact, also be a principal permease involved in transport of the normal purines guanine, hypoxanthine, and/or xanthine. IMPORTANCE:Nucleotide metabolism is a critical aspect of the overall metabolism of the cell, as it is central to the core processes of RNA and DNA synthesis. At the same time, nucleotide metabolism can be subverted by analogs of the normal DNA or RNA bases, leading to highly toxic and mutagenic effects. Thus, understanding how cells process both normal and modified bases is of fundamental importance. This work describes a novel suppressor of the toxicity of certain modified purine bases in the bacterium Escherichia coli. This suppressor encodes a putative high-affinity nucleobase transporter that mediates the import of the modified purine bases. It is also a likely candidate for the long-sought high-affinity importer for the normal purines, like guanine and hypoxanthine. |
| 巻・号 | 4(6) |
| ページ | e00661-13 |
| 公開日 | 2013-10-29 |
| DOI | 10.1128/mBio.00661-13 |
| PII | mBio.00661-13 |
| PMID | 24169576 |
| PMC | PMC3809563 |
| MeSH | Anti-Bacterial Agents / metabolism* Anti-Bacterial Agents / toxicity* Escherichia coli / drug effects* Escherichia coli / enzymology* Escherichia coli / genetics Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism* Mutation Purines / metabolism* Purines / toxicity* |
| IF | 6.784 |
| 引用数 | 7 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | JW4025-KC JW5636-KC JW5467-KC |