RRC ID 35725
著者 Sakane A, Alamir Mahmoud Abdallah A, Nakano K, Honda K, Kitamura T, Imoto I, Matsushita N, Sasaki T.
タイトル Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins.
ジャーナル Genes Cells
Abstract We previously showed that Rab13 and its effector protein, junctional Rab13-binding protein (JRAB)/molecules interacting with CasL-like 2 (MICAL-L2), regulate junctional development by modulating cell adhesion molecule transport and actin cytoskeletal reorganization in epithelial cells. Here, we investigated how JRAB regulates reorganization of the actin cytoskeleton in NIH3T3 fibroblasts, in an attempt to obtain novel insights into the mechanism of JRAB action. To this end, we expressed mutant proteins that adopt a constitutively open or closed state and then examined effect on cellular morphology of the resulting actin cytoskeletal reorganization. Expression of the JRABΔCT mutant (constitutively 'closed' state) induced stress fibers, whereas expression of the JRABΔCC mutant (constitutively 'open' state) caused cell spreading with membrane ruffles. Next, we identified the proteins involved in JRAB-induced rearrangement of actin cytoskeleton leading to morphological changes. In NIH3T3 cells expressing HA-JRABΔCC, filamin, an actin cross-linking protein, coimmunoprecipitated with HA-JRABΔCC. Expression of ASB2 induced degradation of all three filamin isoforms and inhibited the JRABΔCC-induced cell spreading. Consistent with our previous results, actinin-1/-4 were also immunoprecipitated with HA-JRABΔCC. However, actinin-1/-4 have no effect on the cell spreading regulated by JRABΔCC. These data suggest that JRAB contributes to the rearrangement of the actin cytoskeleton during cell spreading via filamins.
巻・号 18(9)
ページ 810-22
公開日 2013-9-1
DOI 10.1111/gtc.12078
PMID 23890175
MeSH Actinin / metabolism Animals Cell Membrane / metabolism Cytoskeletal Proteins / chemistry Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Filamins / metabolism* HEK293 Cells Humans Mice Microfilament Proteins Mutation NIH 3T3 Cells Protein Binding Protein Isoforms / metabolism Protein Structure, Tertiary Stress Fibers / metabolism
IF 1.655
引用数 8
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY
リソース情報
遺伝子材料 pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393).