RRC ID 35740
著者 Yuasa M, Kiuchi T, Banno Y, Katsuma S, Shimada T.
タイトル Identification of the silkworm quail gene reveals a crucial role of a receptor guanylyl cyclase in larval pigmentation.
ジャーナル Insect Biochem Mol Biol
Abstract Diverse color patterns on the integument of lepidopteran larvae play important roles in their survival through camouflage, mimicry, sexual signaling, and aposematism. In the silkworm Bombyx mori, many color pattern variations have been preserved in inbred strains making them a good model for elucidating the molecular mechanisms that underlie color pattern formation. In this study, we focused on the silkworm quail (q) mutant, which exhibits abnormalities in multiple pigment biosynthesis pathways. Positional cloning of the q gene revealed that disruption of a guanylyl cyclase gene, BmGC-I, is responsible for its abnormal pigmentation. In q mutants, we identified a 16-bp deletion in the BmGC-I transcript, resulting in the production of a premature stop codon. Knockout of the BmGC-I gene resulted in the q-like abnormal pigmentation, thereby demonstrating that the BmGC-I gene is involved in the pigment biosynthesis pathway in the integument. Moreover, quantitative reverse transcription polymerase chain reaction showed that BmGC-I was strongly expressed in the fourth instar on day 2. Our results suggest that BmGC-I deficiency affects the pigment biosynthesis pathway, which supports the involvement of guanylyl cyclase in larval coloration.
巻・号 68
ページ 33-40
公開日 2016-1-1
DOI 10.1016/j.ibmb.2015.10.016
PII S0965-1748(15)30063-1
PMID 26561270
MeSH Animals Base Sequence Bombyx / genetics Bombyx / growth & development Bombyx / metabolism Bombyx / physiology* Cloning, Molecular DNA Female Gene Knockout Techniques Genes, Insect Larva / physiology Male Molecular Sequence Data Phenotype Pigmentation* / genetics Quail Receptors, Guanylate Cyclase-Coupled / physiology* Transcriptome
IF 3.827
引用数 7
WOS 分野 ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ c55 b32 k01 k02 m42 d33