RRC ID |
36031
|
著者 |
Hirata T, Amano T, Nakatake Y, Amano M, Piao Y, Hoang HG, Ko MS.
|
タイトル |
Zscan4 transiently reactivates early embryonic genes during the generation of induced pluripotent stem cells.
|
ジャーナル |
Sci Rep
|
Abstract |
The generation of induced pluripotent stem cells (iPSCs) by the forced expression of defined transcription factors in somatic cells holds great promise for the future of regenerative medicine. However, the initial reprogramming mechanism is still poorly understood. Here we show that Zscan4, expressed transiently in2-cell embryos and embryonic stem cells (ESCs), efficiently produces iPSCs from mouse embryo fibroblasts when coexpressed with Klf4, Oct4, and Sox2. Interestingly, the forced expression of Zscan4 is required onlyfor the first few days of iPSC formation. Microarray analysis revealed transient and early induction of preimplantation-specific genes in a Zscan4-dependent manner. Our work indicates that Zscan4 is a previously unidentified potent natural factor that facilitates the reprogramming process and reactivates early embryonic genes.
|
巻・号 |
2
|
ページ |
208
|
公開日 |
2012-1-1
|
DOI |
10.1038/srep00208
|
PII |
srep00208
|
PMID |
22355722
|
PMC |
PMC3250575
|
MeSH |
Animals
Cells, Cultured
Gene Expression Regulation, Developmental / physiology*
Kruppel-Like Factor 4
Kruppel-Like Transcription Factors / genetics*
Mice
Octamer Transcription Factor-3 / genetics*
Oligonucleotide Array Sequence Analysis
Pluripotent Stem Cells / cytology*
SOXB1 Transcription Factors / genetics*
Transcription Factors / physiology*
|
IF |
3.998
|
引用数 |
57
|
WOS 分野
|
CELL BIOLOGY
|
リソース情報 |
遺伝子材料 |
pPyCAG-BstXI-IP (RDB07099) |