RRC ID 36111
著者 Fujii T, Yamamoto K, Banno Y.
タイトル Molybdenum cofactor deficiency causes translucent integument, male-biased lethality, and flaccid paralysis in the silkworm Bombyx mori.
ジャーナル Insect Biochem Mol Biol
Abstract Uric acid accumulates in the epidermis of Bombyx mori larvae and renders the larval integument opaque and white. Yamamoto translucent (oya) is a novel spontaneous mutant with a translucent larval integument and unique phenotypic characteristics, such as male-biased lethality and flaccid larval paralysis. Xanthine dehydrogenase (XDH) that requires a molybdenum cofactor (MoCo) for its activity is a key enzyme for uric acid synthesis. It has been observed that injection of a bovine xanthine oxidase, which corresponds functionally to XDH and contains its own MoCo activity, changes the integuments of oya mutants from translucent to opaque and white. This finding suggests that XDH/MoCo activity might be defective in oya mutants. Our linkage analysis identified an association between the oya locus and chromosome 23. Because XDH is not linked to chromosome 23 in B. mori, MoCo appears to be defective in oya mutants. In eukaryotes, MoCo is synthesized by a conserved biosynthesis pathway governed by four loci (MOCS1, MOCS2, MOCS3, and GEPH). Through a candidate gene approach followed by sequence analysis, a 6-bp deletion was detected in an exon of the B. mori molybdenum cofactor synthesis-step 1 gene (BmMOCS1) in the oya strain. Moreover, recombination was not observed between the oya and BmMOCS1 loci. These results indicate that the BmMOCS1 locus is responsible for the oya locus. Finally, we discuss the potential cause of male-biased lethality and flaccid paralysis observed in the oya mutants.
巻・号 73
ページ 20-6
公開日 2016-6-1
DOI 10.1016/j.ibmb.2016.03.008
PII S0965-1748(16)30036-4
PMID 27041280
MeSH Amino Acid Sequence Animals Base Sequence Bombyx / genetics Bombyx / growth & development Bombyx / physiology* Cloning, Molecular Coenzymes / chemistry Coenzymes / deficiency Coenzymes / genetics* Dioxygenases / genetics Dioxygenases / metabolism Fungal Proteins / genetics Fungal Proteins / metabolism Insect Proteins / chemistry Insect Proteins / deficiency Insect Proteins / genetics* Larva / genetics Larva / growth & development Larva / physiology Male Metalloproteins / chemistry Metalloproteins / deficiency Metalloproteins / genetics* Molybdenum Cofactors Pteridines / chemistry
IF 3.827
引用数 6
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY ENTOMOLOGY
リソース情報
カイコ p50 o36 o46 bmov2p23