| RRC ID |
36470
|
| 著者 |
Nonomura K, Nakano M, Fukuda T, Eiguchi M, Miyao A, Hirochika H, Kurata N.
|
| タイトル |
The novel gene HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1 of rice encodes a putative coiled-coil protein required for homologous chromosome pairing in meiosis.
|
| ジャーナル |
Plant Cell
|
| Abstract |
We have identified and characterized a novel gene, PAIR1 (HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1), required for homologous chromosome pairing and cytokinesis in male and female meiocytes of rice (Oryza sativa). The pair1 mutation, tagged by the endogenous retrotransposon Tos17, exhibited meiosis-specific defects and resulted in complete sterility in male and female gametes. The PAIR1 gene encodes a 492-amino acid protein, which contains putative coiled-coil motifs in the middle, two basic regions at both termini, and a potential nuclear localization signal at the C terminus. Expression of the PAIR1 gene was detected in the early stages of flower development, in which the majority of the sporocytes had not entered meiosis. During prophase I of the pair1 meiocyte, all the chromosomes became entangled to form a compact sphere adhered to a nucleolus, and homologous pairing failed. At anaphase I and telophase I, chromosome nondisjunction and degenerated spindle formation resulted in multiple uneven spore production. However, chromosomal fragmentation frequent in plant meiotic mutants was never observed in all of the pair1 meiocytes. These observations clarify that the PAIR1 protein plays an essential role in establishment of homologous chromosome pairing in rice meiosis.
|
| 巻・号 |
16(4)
|
| ページ |
1008-20
|
| 公開日 |
2004-4-1
|
| DOI |
10.1105/tpc.020701
|
| PII |
tpc.020701
|
| PMID |
15031413
|
| PMC |
PMC412873
|
| MeSH |
Amino Acid Sequence
Base Sequence
Chromosome Pairing
Chromosomes, Plant / genetics
DNA, Plant / genetics
Genes, Plant*
Meiosis / genetics
Molecular Sequence Data
Mutation
Oryza / cytology
Oryza / genetics*
Phenotype
Plant Proteins / chemistry
Plant Proteins / genetics
Protein Structure, Secondary
Spores / genetics
|
| IF |
9.618
|
| 引用数 |
107
|
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| イネ |
Sterile strain (STER-NIG0004 (PAIR1-1), STER-NIG0005 (PAIR1-2), STER-NIG0006 (PAIR1-3)) |
